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Statements

GST binds USP8. 7 / 7
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sparser
"Pull-down assays showed that bacterially purified GST-USP8 bound trichoplein in RPE1 lysates (Fig.  xref )."
29472535
sparser
"Ten microliters of each IVT reaction was incubated with 220 pmoles of purified GST, GST-USP8(1–133), or GST-USP54-MIT(792–868) at 4 °C for an hour in 300 μl assay buffer (20 mM Hepes pH 7.3, 120 mM KO[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19302785
sparser
"GST-USP8-MIT (GST-UBPY(1–133)) has been previously described [14] , as have the yeast two-hybrid and Myc-tagged CHMP constructs [11] ."
19302785
sparser
"To examine whether STAM1 functions as an adaptor linking USP8 to Sec31A, the lysates of cells overexpressing STAM1/2 and Sec31A were subjected to a pull-down assay using GST–USP8 710−1110 ."
29604273
sparser
"GST, GST-USP8(1–133) and GST-USP54-MIT(792–868) were expressed in Rosetta (DE3) pLysS bacteria (Novagen) and purified with glutathione sepharose (Amersham) according to the manufacturer’s instructions[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19302785
reach
"GST-Gads, but not GST alone, bound to UBPY, Gab2, and BLNK."
12176364
sparser
"GST‐USP8 was purified with a BeyGoldTM GST‐tag Purification Kit (Beyotime) according to the manufacturer's instructions."
40525588