IndraLab

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OTUB1 deubiquitinates S9. 5 / 5
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"OTUB1 deubiquitinates NS2 in cells."
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"Considering that OTUB1 is a deubiquitinase [26,27], we asked if OTUB1 deubiquitinates and stabilizes NS2."
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"We found that although levels of NS2 conjugated with V5-Ub were substantially reduced if HEK293T cells were cotransfected with pFlag-OTUB1 (Fig 5B, lanes 3, 4), no reduction of Ub-HA-NS2 was observed if cells were cotransfected with pFlag-mOTUB1 (Fig 5B, lane 5), indicating that OTUB1 deubiquitinates NS2 through its catalytic activity."
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"To confirm that OTUB1 can deubiquitinate NS2 in vitro, we immunoprecipitated HA-NS2 from HEK293T cells that were transfected with pV5-Ub or pHA-NS2, or cotransfected with both plasmids."
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"Transfection of pFlag-OTUB1 was shown to increase the stability of HA-NS2 (Fig 7), thereby confirming that OTUB1 can deubiquitinate and stabilize NS2."
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