IndraLab

Statements


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"In contrast, cells exposed to viscumin in the presence of 2-deoxyglucose and NaN3, which inhibit endocytosis, were not intoxicated when the cells were treated with antiviscumin and then transferred to normal medium."

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"Endocytosis inhibitors, such as CPZ, CQ, Mβ-CD, NaN , CyD, EIPA and heparin, inhibit these factors to suppress endocytic pathways."

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"To understand of the mechanism of the cellular internalization of FPLM NPs, various endocytosis inhibitors (including 10 mM NaN , 10 μg/mL CPZ, 2.5 μg/mL genistein, 25 μg/mL colchicine, and 10 μg/mL nystatin) were introduced."

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"To further investigate the mechanism of cellular internalization of CeONZs in human cells, we treated H9-CMs with NaN and 2-DG to inhibit energy-mediated endocytosis [50] prior to the addition of 60 μM of CeONZs."

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"Mβ-CD suppresses pinocytosis through caveolin, NaN disrupts endocytosis by inhibiting mitochondrial ATP production and CyD and EIPA are inhibitors of macropinocytosis."

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"First, co-incubation with NaN , a disruptor of adenosine triphosphate, decreased the uptake of EXOs to 78% (P < 0.05, Fig. 4C), indicating the involvement of energy-dependent endocytosis."

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"Then, the cells were pretreated with different endocytosis inhibitors including Chlorpromazine hydrochloride (CPZ;10 µg/mL), Glucose (0.45 M), Filipin III (5 µg/mL), Genistein (0.2 mM), Cytochalasin D (3 µM), Amiloride Hydrochloride (DMA; 10 µM), NaN (1 mg/mL), and Monensin (3 μM)."

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"In order to inhibit all pathways of endocytosis, MDA-MB-231 cells were pretreated with sodium azide (NaN ) (10 mM) and 2-Deoxyglucose (DOG) (5 mM) for 30 min and the peptide was added to the wells."

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"We found that both NaN and incubation at 4°C substantially reduced the efficiency of cellular internalization of nucleosomes (Figure 2A–C), indicating that the majority of cellular internalization of nucleosomes involves energy-dependent endocytosis."