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JOSD2 binds IMPDH2. 13 / 13
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"It shows that JOSD2 binds directly to the C-terminal catalytic domain of IMPDH2 and cleaves the K63-linked polyubiquitin of IMPDH2 at K134."
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"Therefore, the potential JOSD2–IMPDH2 interaction, which probably regulates NF- κ B signal and inflammatory response in IBD, aroused our interest."
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"Finally, the interaction between endogenous JOSD2 and IMPDH2 in mouse primary bone marrow-derived macrophages (BMDMs) was confirmed by Co-IP assay under physiological conditions, and the interaction between JOSD2 and IMPDH2 was further increased when the BMDMs were challenged by lipopolysaccharide (LPS) (Fig. 5H)."
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"JOSD2 directly interacts with an inflammation-regulating protein IMPDH2."
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"Then, co-immunoprecipitation (Co-IP) and double immunofluorescence staining experiments further confirmed the interaction between JOSD2 and IMPDH2 via in vitro co-transfection of JOSD2-Flag and GFP-IMPDH2-HA plasmids ( xref D and E)."
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"Collectively, these data confirm the direct interaction between JOSD2 and IMPDH2."
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"To further explore the essential domain of IMPDH2 that interacts with JOSD2, a series of GFP-IMPDH2-HA truncated mutants were constructed to identify the key domain in IMPDH2 protein ( xref F)."
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"The present work shows that JOSD2 directly interacts with IMPDH2 and, via K63-linked deubiquitination, reduces IMPDH2 activity in macrophages."
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"As shown in the xref , the interaction between JOSD2 and IMPDH2-K134R was decreased in macrophages."
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"Interestingly, the LC–MS/MS analysis and immunoprecipitation assay showed that JOSD2 could interact with IMPDH2, an important inflammation-regulating protein ( xref B and C)."
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"Finally, the interaction between endogenous JOSD2 and IMPDH2 in mouse primary bone marrow-derived macrophages (BMDMs) was confirmed by Co-IP assay under physiological conditions, and the interaction between JOSD2 and IMPDH2 was further increased when the BMDMs were challenged by lipopolysaccharide (LPS) ( xref H)."
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"Then, co-immunoprecipitation (Co-IP) and double immunofluorescence staining experiments further confirmed the interaction between JOSD2 and IMPDH2 via in vitro co-transfection of JOSD2-Flag and GFP-IMPDH2-HA plasmids (Fig. 5D and E)."
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"Additionally, endogenous JOSD2 and IMPDH2 also interacted with each other in the mouse colon tissues ( xref )."
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