IndraLab

Statements

JOSD2 binds IMPDH2. 13 / 13
| 2 11
sparser
"Interestingly, the LC–MS/MS analysis and immunoprecipitation assay showed that JOSD2 could interact with IMPDH2, an important inflammation-regulating protein ( xref B and C)."
40177575
sparser
"Finally, the interaction between endogenous JOSD2 and IMPDH2 in mouse primary bone marrow-derived macrophages (BMDMs) was confirmed by Co-IP assay under physiological conditions, and the interaction between JOSD2 and IMPDH2 was further increased when the BMDMs were challenged by lipopolysaccharide (LPS) ( xref H)."
40177575
sparser
"JOSD2 directly interacts with an inflammation-regulating protein IMPDH2."
40177575
sparser
"Then, co-immunoprecipitation (Co-IP) and double immunofluorescence staining experiments further confirmed the interaction between JOSD2 and IMPDH2 via in vitro co-transfection of JOSD2-Flag and GFP-IMPDH2-HA plasmids ( xref D and E)."
40177575
sparser
"Collectively, these data confirm the direct interaction between JOSD2 and IMPDH2."
40177575
reach
"Then, co-immunoprecipitation (Co-IP) and double immunofluorescence staining experiments further confirmed the interaction between JOSD2 and IMPDH2 via in vitro co-transfection of JOSD2-Flag and GFP-IMPDH2-HA plasmids (Fig. 5D and E)."
40177575
sparser
"Additionally, endogenous JOSD2 and IMPDH2 also interacted with each other in the mouse colon tissues ( xref )."
40177575
sparser
"It shows that JOSD2 binds directly to the C-terminal catalytic domain of IMPDH2 and cleaves the K63-linked polyubiquitin of IMPDH2 at K134."
40177575
sparser
"Therefore, the potential JOSD2–IMPDH2 interaction, which probably regulates NF- κ B signal and inflammatory response in IBD, aroused our interest."
40177575
reach
"Finally, the interaction between endogenous JOSD2 and IMPDH2 in mouse primary bone marrow-derived macrophages (BMDMs) was confirmed by Co-IP assay under physiological conditions, and the interaction between JOSD2 and IMPDH2 was further increased when the BMDMs were challenged by lipopolysaccharide (LPS) (Fig. 5H)."
40177575
sparser
"To further explore the essential domain of IMPDH2 that interacts with JOSD2, a series of GFP-IMPDH2-HA truncated mutants were constructed to identify the key domain in IMPDH2 protein ( xref F)."
40177575
sparser
"The present work shows that JOSD2 directly interacts with IMPDH2 and, via K63-linked deubiquitination, reduces IMPDH2 activity in macrophages."
40177575
sparser
"As shown in the xref , the interaction between JOSD2 and IMPDH2-K134R was decreased in macrophages."
40177575