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SLC7A11 binds OTUB1. 139 / 139
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"The direct interaction between ubiquitin hydrolase ovarian tumor domain protease domain, ubiquitin aldehyde binding protein 1 (OTUB1) and SLC7A11 stabilizes the SLC7A11 protein, and OTUB1 knockdown triggers SLC7A11 expression-dependent ferroptosis (13)."
36937406
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"Studies have shown that CD44 promotes the deubiquitination of SLC7A11 by promoting the binding of the deubiquitination enzyme OTUB1 to SLC7A11, thus making SLC7A11 on the cell membrane stable against proteasome pathway degradation [ xref ]."
36672372
biogrid
No evidence text available
33878705
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"CD44, serving as an adhesive molecule in cancer stem cells, has a variant, CD44v, that ensures the stability of SLC7A11 protein by adjusting interactions between OTUB1 and SLC7A11 (51)."
38515565
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"The interaction between OTUB1 and SLC7A11 offers a novel perspective for treating gliomas."
41331230
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"As m6A eraser, FTO mainly plays demethylation activity for the m6A of single‐stranded RNA, thus regulating gene expression. xref , xref Huang et al. xref revealed that FTO suppressed nasopharyngeal carcinoma cell ferroptosis to enhance radioresistance via stabilizing the interaction between OTUB1 and SLC7A11 by inhibiting the m6A modification of OTUB1."
39739936
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"Next, to verify the interaction between SLC7A11 and OTUB1, rat PASMCs were transfected with pcDNA3.1-Flag-SLC7A11 or pcDNA3.1-Flag-OTUB1 expression vectors, and the Co-IP assays of SLC7A11 and OTUB1 w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35700835
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"Additionally, OTUB1 also directly interacts with and stabilizes xCT, while the inhibitory effects of CD44 on ferroptosis are partly attributed to the enhancement of the OTUB1-xCT interaction (59, 60)."
38464226
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"The inhibition of OTUB1 on ferroptosis depends on the interaction between OTUB1 and SLC7A11 [17]."
37714846
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"28 , 29 Huang et al. 30 revealed that FTO suppressed nasopharyngeal carcinoma cell ferroptosis to enhance radioresistance via stabilizing the interaction between OTUB1 and SLC7A11 by inhibiting the m6A modification of OTUB1."
39739936
sparser
"The newest research indicated that CD44 played important roles that could stabilize SLC7A11 by increasing the recruitment of OTUB1 and promotes the interaction of SLC7A11 with OTUB1 , thereby inhibiting the ferroptosis in tumor cells (Liu et al., xref )."
33869286
sparser
"For instance, the deubiquitinating enzyme OTUB1 directly interacts with SLC7A11 to inhibit its ubiquitination, thereby stabilizing SLC7A11 and suppressing ferroptosis in cancer cells [ xref ]."
40825027
sparser
"By interacting with OTUB1 and SLC7A11 , Circ-BGN promotes deubiquitination and prevents ferroptosis."
38887390
biogrid
No evidence text available
35234341
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"Wang et al. found that circBGN binds and enhances the interaction between OTUB1 and SLC7A11, stabilizing SLC7A11 protein and inhibiting ferroptosis, leading to trastuzumab resistance in HER2-positive BC xref ."
39825074
sparser
"However, the specific mechanisms by which Ferrostatin-1 interacted with OTUB1 and SLC7A11 to modulate podocyte function have not been elucidated to date."
39500879
sparser
"To validate the in vivo interaction between SLC7A11 and OTUB1, we first transfected native H1299 cells with an OTUB1 expression vector in the presence or absence of a vector encoding Flag-tagged SLC7A11."
30709928
sparser
"To ascertain whether OTUB1 and SLC7A11 interact directly, we performed in vitro GST pull-down assays by incubating a GST-fusion protein containing full-length OTUB1 with purified Flag-SLC7A11."
30709928
sparser
"Notably, overexpression of the cancer stem cell marker CD44 enhanced the stability of SLC7A11 by promoting the interaction between SLC7A11 and OTUB1; depletion of CD44 partially abrogated this interaction."
30709928
sparser
"We found that OTUB1 directly interacts with SLC7A11 and regulates SLC7A11 stability in a p53-independent manner."
30709928
sparser
"Loss of OTUB1 suppresses tumor growth in xenograft mouse models but these effects are largely abrogated by SLC7A11 overexpression, validating a critical role for the OTUB1-SLC7A11 interaction in tumorigenesis."
30709928
sparser
"Finally, the SLC7A11-OTUB1 interaction is also regulated by CD44, a key marker of cancer stem cells."
30709928
sparser
"Moreover, as shown in xref , co-immunoprecipitation assays revealed that both functional mutants OTUB1(C91A) and OTUB1(D88A) can interact with SLC7A11 although the binding affinity between OTUB1(D88A) and SLC7A11 was apparently lower than the one between OTUB1(C91A) and SLC7A11."
30709928
sparser
"OTUB1 is overexpressed in human cancers and the OTUB1-SLC7A11 interaction is critical for tumor growth."
30709928
sparser
"The OTUB1-SLC7A11 interaction is tightly regulated by CD44 in human cancer cells."
30709928
sparser
"To further elucidate the significance of SLC7A11 stability control in tumorigenesis, we searched for potential regulation of the OTUB1-SLC7A11 interaction by other factors in human cancers."
30709928
sparser
"To elucidate the molecular mechanism that underlies this effect, we examined whether CD44 modulates the SLC7A11-OTUB1 interaction by co-immunoprecipitation analysis."
30709928
sparser
"To avoid the issue that increased SLC7A11 protein levels are the only factor for the increase of the OTUB1-SLC7A11 interaction, we normalized the amounts of SLC7A11 after immunoprecipitation (IP: lanes 1 and 2 vs. lanes 3 and 4, xref )."
30709928
sparser
"Taken together, these data demonstrate that CD44 can enhance the OTUB1-SLC7A11 interaction even with the same amount of SLC7A11 protein."
30709928
sparser
"Our data showed that CD44 can interact with SLC7A11 while SLC7A11 also interacts with OTUB1."
30709928
sparser
"In particular, our results show that (i) OTUB1 is a bona fide binding partner of SLC7A11 both in vitro and in vivo; (ii) OTUB1 acts as a major regulator for SLC7A11 activity in human cancer cells; (iii) OTUB1 inactivation promotes ferroptosis in human cancer cells primarily by down-regulating SLC7A11 levels; (iv) OTUB1 is overexpressed in human cancers and the OTUB1-SLC7A11 interaction is critical for tumor growth; (v) The OTUB1-SLC7A11 interaction is tightly regulated by CD44 in human cancer cells."
30709928
sparser
"These data demonstrate that the SLC7A11-OTUB1 interaction is modulated by CD44 in human cancer cells and that OTUB1 is required for CD44-mediated suppression of ferroptosis in human cancer cells."
30709928
sparser
"Interestingly, by promoting the interaction between SLC7A11 and OTUB1, the CD44 cellular adhesion molecule can also enhance SLC7A11 stability and inhibit ferroptosis."
30709928
sparser
"It will be interesting to know whether the OTUB1-SLC7A11 interaction is regulated by this modification ( xref )."
30709928
sparser
"In this study, we demonstrate that, by promoting the interaction between SLC7A11 and OTUB1, CD44 serves as a positive regulator of SLC7A11 activity by facilitating the recruitment of OTUB1 and thereby reducing the sensitivity of cancer cells to oxidative stress and ferroptotic death ( xref and xref )."
30709928
sparser
"To further define the functional domain of the ZRANB1 E3 ligase for SLC7A11, we transfected full-length ZRANB1 and three truncated forms ( xref ) into HEK293T cells ( xref ) and found that the OTU domain not only interacted with SLC7A11 ( xref ) but was also required for the degradation and ubiquitination of SLC7A11 by ZRANB1 ( xref )."
37831441
sparser
"OTUB1 interacts directly with SLC7A11, stabilizing its expression by preventing its ubiquitination, which is essential for GSH synthesis."
40822033
sparser
"CD44, serving as an adhesive molecule in cancer stem cells, has a variant, CD44v, that ensures the stability of SLC7A11 protein by adjusting interactions between OTUB1 and SLC7A11 ( xref )."
38515565
sparser
"Notably, overexpression of the cancer-stem-cell-marker CD44 enhanced the stability of SLC7A11 by promoting the interaction between SLC7A11 and OTUB1; the depletion of CD44 partially abrogated this interaction [ xref ]."
33540700
sparser
"The cell surface protein CD44 enhances the interaction between OTUB1 and SLC7A11, thereby preventing ferroptosis. xref These findings suggest that OTUB1 is crucial for regulating glioma cell progression, primarily through its direct interaction with the xCT system and modulation of ferroptosis pathways."
40822033
sparser
"CircBGN promotes ferroptosis by increasing the interaction between OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1) and SLC7A11 and the level of SLC7A11 protein."
39022677
sparser
"Want et al. additionally determined that BC cells resistant to trastuzumab exhibit the upregulation of circ-BGN, which contributes to this chemoresistance through its ability to directly bind OTUB1 and SLC7A11, thus inhibiting ferroptotic cell death via the enhancement of OTUB1-mediated SLC7A11 deubiquitination ( xref )."
37941557
sparser
"To evaluate the OTUB1-SLC7A11 interaction under physiological conditions, we performed co-IP assays in glioma cells."
34927544
reach
"CD44 expression enhances the stability of SLC7A11 and suppresses ferroptosis by promoting the direct interaction between the ubiquitin hydrolase otubain-1 (OTUB1) and SLC7A11."
36552652
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"By stabilizing the interaction between OTUB1 and SLC7A11, FTO inhibits radiation-induced cell ferroptosis and ultimately triggers radiotherapy resistance in nasopharyngeal carcinoma (Huang et al., 2023)."
38550378
sparser
"OTUB1, which is overexpressed in multiple human cancers, directly interacts with SLC7A11 to remove its ubiquitination marks, preventing proteasome-mediated degradation and increasing its stability, thus inhibiting the activation of ferroptosis."
39769177
sparser
"Furthermore, overexpression of the cancer stem cell marker CD44 promotes the interaction between OTUB1 and SLC7A11, reducing SLC7A11 degradation and inhibiting ferroptosis."
39769177
reach
"Wang et al. found that circBGN binds and enhances the interaction between OTUB1 and SLC7A11, stabilizing SLC7A11 protein and inhibiting ferroptosis, leading to trastuzumab resistance in HER2-positive BC ."
39825074
sparser
"CD44v, a surface molecular marker of CSCs, enhances SLC7A11 protein stability by maintaining the interaction of the ubiquitin hydrolase OTUB1 with SLC7A11 ( Liu et al., 2019 ), thereby establishing th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37598896
sparser
"Notably, since CD44, another CSC marker, has been shown to be essential for the OTUB1-SLC7A11 interaction, we also detected the effects of OTUB1 on CD44 expression and found that OTUB1 had no efffect on CD44 protein expression xref ."
34927544
sparser
"DUB OTUB1 can also directly interact with SLC7A11 and stabilize SLC7A11 in a TP53-independent manner."
36551253
sparser
"For instance, previous study indicated that OTUB1 directly interacted with SLC7A11, which facilitated CD44-mediated effects on ferroptosis in human cancers [ xref ]."
35354355
sparser
"Stem cell marker CD44 expression suppresses ferroptosis in cancer cells in an OTUB1-dependent manner by promoting the interaction between SLC7A11 and OTUB1 [ xref ]."
36551253
sparser
"The deubiquitinase, OTUB1, directly interacts with SLC7A11 and stabilizes the SLC7A11 protein at the post-translational level, leading to the upregulation of SLC7A11 [ xref ]."
38042059
sparser
"Additionally, co-IP analysis indicated that OTUB1 directly interacted with SLC7A11 in glioma cells."
34927544
sparser
"Together, these results indicated that endogenous H 2 S could increase the binding of OTUB1 with xCT by persulfidating OTUB1 at cysteine 91 to regulate the stability of xCT."
33878705
sparser
"To delineate the cysteine residue accounted for the effect of H 2 S on the binding of OTUB1 with xCT."
33878705
sparser
"The decreased xCT levels and persulfidation mediated regulation of OTUB1 binding with xCT was also validated in vivo ."
33878705
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"OTUB1, a member of the OTU protease subfamily, directly interacts with and stabilizes SLC7A11 in cancer cells by preventing its ubiquitination (22)."
37703360
sparser
"Circ-BGN was found to directly bind to OTUB1 and SLC7A11 and enhance OTUB1-mediated deubiquitination of SLC7A11, thereby inhibiting ferroptosis ( xref )."
37332354
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"Two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) showed that SLC7A11, OTUB1, and SLC3A2 were detected in association with the ~150-kDa complex (Fig. 2B), and co-immunoprecipitation analysis further confirmed the association between SLC7A11, SLC3A2, and OTUB1 after IL-1β stimulation (Fig. 2C)."
37703360
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"Although the transmembrane glycoprotein, CD44, is also known to bind and stabilize the SLC7A11 and OTUB1 complex (22), we were not able to detect CD44 in primary lung epithelial cells in the absence or presence of IL-1β stimulation.PSSG is a key regulator of biological responses induced by redox perturbations."
37703360
sparser
"We wonder whether this modification is involved in the OTUB1-SLC7A11 interaction in glioma cells."
34927544
sparser
"By stabilizing the interaction between OTUB1 and SLC7A11, FTO inhibits radiation-induced cell ferroptosis and ultimately triggers radiotherapy resistance in nasopharyngeal carcinoma ( xref )."
38550378
reach
"Immunoprecipitation of hemagglutinin (HA)–tagged SLC7A11 or OTUB1 shows a constitutive interaction between SLC7A11, SLC3A2, and OTUB1 (Fig. 5A)."
37703360
reach
"Stabilization of SLC7A11 by OTUB1 has been speculated to involve direct binding between OTUB1 and SLC7A11 as well as the inhibition of E2 enzymes (22)."
37703360
biogrid
No evidence text available
35494004
sparser
"Interestingly, CD44 demonstrated the ability to stabilize xCT and OTUB1 interaction in an OTUB1-dependent manner."
40114966
biogrid
No evidence text available
35494004
sparser
"Although the direct interaction of CD44 and OTUB1 is much lower in comparison to OTUB1-xCT interaction, nevertheless, this three-protein complex is thought to stabilize xCT more effectively, possibly further enhancing chemoresistance."
40114966
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"The interaction between OTUB1 and SLC7A11 can be enhanced by CD44, a transmembrane glycoprotein receptor that plays an important role in tumor initiation, progression, and metastasis [ 102 ]."
30880243
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"It remains unknown whether the OTUB1-SLC7A11 complex is regulated by the BECN1-SLC7A11 complex in ferroptosis [ 101 ]."
30880243
sparser
"In this study, we investigated how 2‐deoxy‐ d ‐ribose (dRib) induces ferroptosis in β‐cells and identified the OTUB1–SLC7A11 axis as a critical regulatory mechanism."
41077887
sparser
"These findings highlight the OTUB1–SLC7A11 axis as a novel regulatory pathway of β‐cell ferroptosis and a potential therapeutic target in diabetes."
41077887
sparser
"Notably, overexpression of the cancer stem cell marker CD44 enhances the OTUB1-SLC7A11 interaction to foster ferroptosis resistance. xref "
41550777
sparser
"To our knowledge, this is the first study to demonstrate that the OTUB1‐SLC7A11 axis is a key regulatory pathway for ferroptosis in pancreatic β‐cells."
41077887
sparser
"Wang et al. Discovered that Circ-BGN can directly bind to SLC7A11 and OTUB1, a deubiquitinating enzyme that increases SLC7A11 stability."
38408962
reach
"However, one study found that the tumor stem cell marker CD44 inhibited ferroptosis in tumor cells in a manner dependent on the deubiquitinase OTUB1, and overexpression of CD44 improved the stability of the SLC7A11 protein by promoting the interaction between SLC7A11 and OTUB1 (84)."
36185243
reach
"OTUB1, a ubiquitin hydrolase, could directly bind to SLC7A11 and strengthen the stability of the latter, then reduce the sensitivity of cells to ferroptosis [ 31 ]."
34147710
sparser
"The deubiquitinating enzyme OTUB1 interacts with the N-terminal domain of SLC7A11 to prevent it from degradation [ xref ]."
35502211
sparser
"CD44 can modulate its protein stability and enhance the OTUB1-SLC7A11 interaction."
35502211
biogrid
No evidence text available
35700835
sparser
"Co‐immunoprecipitation assays confirmed that SLC7A11 interacted with OTUB1, but not with TRIM26 (Figure  xref )."
41077887
reach
"Gu et al. revealed that overexpression of the cancer stem cell marker CD44 promotes the interaction between SLC7A11 and OTUB1."
36612069
sparser
"These findings suggest that targeting the OTUB1–SLC7A11 axis may preserve β‐cell survival and offer a potential therapeutic strategy for diabetes and possibly cancer."
41077887
sparser
"The inhibition of OTUB1 on ferroptosis depends on the interaction between OTUB1 and SLC7A11 [ xref ]."
37714846
sparser
"The deubiquitinase OTUB1 directly interacts with SLC7A11 to stabilize its expression by inhibiting ubiquitin-mediated degradation, thereby suppressing ferroptosis in tumor cells."
41327191
sparser
"Notably, the tumor stem cell marker CD44 facilitates the SLC7A11-OTUB1 interaction to regulate ferroptosis in an OTUB1-dependent manner [ xref , xref ]."
41327191
sparser
"This study showed that FTO overexpression could stabilize the interaction between OTUB1 and SLC7A11, indicating that FTO inhibited iron death through the OTUB1/SLC7A11 pathway [ xref ]."
37522921
sparser
"Given that SLC7A11 protein stability is regulated by the deubiquitylase OTUB1 in a CD44-dependent manner, with CD44 serving as a critical mediator of the OTUB1-SLC7A11 interaction [ xref , xref ], we investigated the expression patterns of these regulatory proteins alongside SLC7A11 and GPX4."
40563331
sparser
"A recent study shows that OTUB1 directly interacts with SLC7A11 and prevents SLC7A11 degradation in cancer cells in a TP53-independent manner [ 102 ]."
30880243
sparser
"CircBGN directly binds to OTUB1 and SLC7A11, enhancing OTUB1-mediated SLC7A11 deubiquitination to inhibit ferroptosis in breast cancer [ xref ]."
37686142
sparser
"Additionally, OTUB1 also directly interacts with and stabilizes xCT, while the inhibitory effects of CD44 on ferroptosis are partly attributed to the enhancement of the OTUB1-xCT interaction ( xref , xref )."
38464226
sparser
"For example, noncanonical deubiquitinase OTU domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) interacts with SLC7A11 to prevent its degradation [ xref ] and the adhesion molecule CD44 variant (CD44v) acts as a binding partner for stabilizing SLC7A11 [ xref ]."
37714846
sparser
"This is the first report implicating the OTUB1‐SLC7A11 axis in β‐cell ferroptosis."
41077887
sparser
"The interaction between OTUB1 and SLC7A11 can be enhanced by CD44, a transmembrane glycoprotein receptor that plays an important role in tumor initiation, progression, and metastasis [ 102 ]."
30880243
sparser
"OTUB1, a ubiquitin hydrolase, could directly bind to SLC7A11 and strengthen the stability of the latter, then reduce the sensitivity of cells to ferroptosis [ 31 ]."
34147710
sparser
"It remains unknown whether the OTUB1-SLC7A11 complex is regulated by the BECN1-SLC7A11 complex in ferroptosis [ 101 ]."
30880243
reach
"Interestingly, CD44 demonstrated the ability to stabilize xCT and OTUB1 interaction in an OTUB1-dependent manner."
40114966
sparser
"Although the transmembrane glycoprotein, CD44, is also known to bind and stabilize the SLC7A11 and OTUB1 complex ( xref ), we were not able to detect CD44 in primary lung epithelial cells in the absence or presence of IL-1β stimulation."
37703360
sparser
"Overexpression of the cancer stem cell marker CD44 increases the stability of SLC7A11 by promoting the interaction between SLC7A11 and OTUB1 [ xref ]."
32516941
sparser
"In this study, Liu and colleagues showed that SLC7A11, OTUB1, and CD44 form a tri-protein complex and that CD44 promotes both SLC7A11-OTUB1 interaction and SLC7A11 protein stability."
30987975
sparser
"CD44 overexpression significantly increased SLC7A11 protein levels and inhibited erastin-induced ferroptosis, but these effects were abrogated in OTUB1 KO cells, suggesting that CD44 regulates SLC7A11 protein stability and ferroptosis through OTUB1 and likely by promoting SLC7A11-OTUB1 interaction."
30987975
reach
"Stem cell marker CD44 expression suppresses ferroptosis in cancer cells in an OTUB1-dependent manner by promoting the interaction between SLC7A11 and OTUB1 [90]."
36551253
sparser
"A key finding of our study is the identification of the OTUB1-SLC7A11 axis as a critical mediator of 4-OI’s protective effects."
40563331
sparser
"Our study primarily reveals the novel OTUB1-SLC7A11 regulatory axis as a key mechanism underlying 4-OI-mediated protection against oxidative stress and ferroptosis."
40563331
reach
"Then, Co-Immunoprecipitation (CO-IP) assays showed that OTUB1 indeed interacts with SLC7A11 and highly sensitive Duolink in situ PLA assays were performed to reveal binding between SLC7A11 and OTUB1 in PANC-1 and CFPAC-1 cells (Figures 4E, F)."
35494004
reach
"Liu et al. (39) found that OTUB1 directly interacts with and stabilizes SLC7A11, and that inactivation of OTUB1 destabilized SLC7A11, leading to inhibition of tumor xenograft growth in mice, which was associated with reduced activation of ferroptosis."
35494004
reach
"Besides, CO-IP and Duolink PLA assays confirmed the interactions between OTUB1 and SLC7A11 and the deubiquitinating effects of OTUB1 on SLC7A11 in PANC-1 and CFPAC-1 cells.As a form of cell death that is associated with iron-dependent peroxidation of polyunsaturated phospholipids in cell membranes, ferroptosis is generally induced through two pathways."
35494004
sparser
"In conclusion, our study reveals that 4-OI protects against APAP-induced hepatotoxicity through a sophisticated network of interacting pathways, centered on the OTUB1-SLC7A11 axis and modulated by Nrf2 signaling."
40563331
reach
"Notably, overexpression of the cancer-stem-cell-marker CD44 enhanced the stability of SLC7A11 by promoting the interaction between SLC7A11 and OTUB1; the depletion of CD44 partially abrogated this interaction [XREF_BIBR]."
33540700
reach
"72 OTUB1 could directly interact with and stabilize SLC7A11, leading to ferroptosis resistance."
38124786
sparser
"However, one study found that the tumor stem cell marker CD44 inhibited ferroptosis in tumor cells in a manner dependent on the deubiquitinase OTUB1, and overexpression of CD44 improved the stability of the SLC7A11 protein by promoting the interaction between SLC7A11 and OTUB1 ( xref )."
36185243
reach
"This study showed that FTO overexpression could stabilize the interaction between OTUB1 and SLC7A11, indicating that FTO inhibited iron death through the OTUB1/SLC7A11 pathway [68]."
37522921
sparser
"Recent studies have shown that overexpression of CD44 in cancer cells can promote the interaction between OTUB1 and SLC7A11 to enhance the stability of SLC7A11 (a subunit of glutamate-cystine antiporter system Xc-) and thereby inhibiting the ferroptosis, while the partial depletion of CD44 abolished this interaction ( xref )."
35155251
sparser
"Then, Co-Immunoprecipitation (CO-IP) assays showed that OTUB1 indeed interacts with SLC7A11 and highly sensitive Duolink ® in situ PLA assays were performed to reveal binding between SLC7A11 and OTUB1 in PANC-1 and CFPAC-1 cells ( xref )."
35494004
sparser
"Gu et al. revealed that overexpression of the cancer stem cell marker CD44 promotes the interaction between SLC7A11 and OTUB1."
36612069
reach
"OTUB1 directly interacted with and stabilized SLC7A11; conversely, OTUB1 knockdown diminished SLC7A11 levels in cancer cells."
30709928
reach
"Notably, overexpression of the cancer stem cell marker CD44 enhanced the stability of SLC7A11 by promoting the interaction between SLC7A11 and OTUB1; depletion of CD44 partially abrogated this interaction."
30709928
sparser
"For instance, ovarian tumor-related proteases (OTU) DUB and ubiquitin aldehyde binding 1 (OTUB1) directly interact with SLC7A11 to stabilize its inhibition of ferroptosis."
38650929
reach
"We found that OTUB1 directly interacts with SLC7A11 and regulates SLC7A11 stability in a p53 independent manner."
30709928
sparser
"Notably, the deubiquitylase OTUB1, CD44, and SLC7A11 form a trimeric complex and the overexpression of CD44 enhanced the stability of SLC7A11 by promoting SLC7A11-OTUB1 interaction."
36084707
reach
"To validate the in vivo interaction between SLC7A11 and OTUB1, we first transfected native H1299 cells with an OTUB1 expression vector in the presence or absence of a vector encoding Flag tagged SLC7A11."
30709928
reach
"Furthermore, overexpression of the cancer stem cell marker CD44 promotes the interaction between OTUB1 and SLC7A11, reducing SLC7A11 degradation and inhibiting ferroptosis."
39769177
sparser
"Besides, CO-IP and Duolink ® PLA assays confirmed the interactions between OTUB1 and SLC7A11 and the deubiquitinating effects of OTUB1 on SLC7A11 in PANC-1 and CFPAC-1 cells."
35494004
reach
"Taken together, although the precise mechanism by which OTUB1 induces SLC7A11 stabilization requires further elucidation, it is very likely that the binding between OTUB1 and SLC7A11 as well as OTUB1 's ability of inhibiting E2 conjugating enzymes recruited by the unknown E3 ligase contribute to SLC7A11 stabilization induced by OTUB1."
30709928
reach
"Our data showed that CD44 can interact with SLC7A11 while SLC7A11 also interacts with OTUB1."
30709928
reach
"Since CD44 can stabilize SLC7A11 by enhancing the binding between SLC7A11 and OTUB1 (XREF_FIG), it is very likely that CD44, OTUB1 and SLC7A11 form a stable three protein complex that leads to more effective stabilization of SLC7A11."
30709928
reach
"Interestingly, by promoting the interaction between SLC7A11 and OTUB1, the CD44 cellular adhesion molecule can also enhance SLC7A11 stability and inhibit ferroptosis."
30709928
reach
"In this study, we demonstrate that, by promoting the interaction between SLC7A11 and OTUB1, CD44 serves as a positive regulator of SLC7A11 activity by facilitating the recruitment of OTUB1 and thereby reducing the sensitivity of cancer cells to oxidative stress and ferroptotic death (XREF_FIG and XREF_FIG)."
30709928
reach
"However, the association between OTUB1 and SLC7A11 in PAH remains obscure."
35700835
reach
"Next, to verify the interaction between SLC7A11 and OTUB1, rat PASMCs were transfected with pcDNA3.1-Flag-SLC7A11 or pcDNA3.1-Flag-OTUB1 expression vectors, and the Co-IP assays of SLC7A11 and OTUB1 w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35700835
biogrid
No evidence text available
30709928
biogrid
No evidence text available
30709928
sparser
"The deubiquitinating enzyme OTUB1 interacts with SLC7A11 to cause SLC7A11 degradation [ 300 ]."
36965540
biogrid
No evidence text available
30709928
biogrid
No evidence text available
30709928
sparser
"CD44 expression enhances the stability of SLC7A11 and suppresses ferroptosis by promoting the direct interaction between the ubiquitin hydrolase otubain-1 (OTUB1) and SLC7A11."
36552652
sparser
"Complementary approaches leverage plant-derived compounds: curcumin suppresses colon cancer angiogenesis through OTUB1 downregulation and subsequent TGFB1/VEGF axis inhibition, xref erianin impedes esophageal squamous cell carcinoma metastasis by triggering OTUB1 ubiquitin-proteasomal degradation, xref solasonine disrupts pancreatic cancer progression via coordinated OTUB1-SLC7A11 destabilization. xref "
41550777