IndraLab

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PRMT1 methylates KCNQ2. 6 / 6
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"To test whether Prmt1 methylates KCNQ2 in vivo, HEK293T cells expressing HA-KCNQ2 were transfected with control or Prmt1 shRNA, followed by immunoprecipitation with anti-KCNQ2 antibodies and immunoblotting with antibodies to asymmetric dimethylarginine (ADMA) and KCNQ2."
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"To determine whether Prmt1 methylates KCNQ2, we performed in vitro methylation assays using various bacterially purified GST-Prmts (Prmt1-6) or myc tagged Prmt5 purified from HEK293T cells and GST-KCNQ2 (amino acids 320-449) that contain all of four arginine residues identified by mass spectrometry."
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"Findings from Kim et al. suggest that arginine methylation of the potassium voltage-gated channel subfamily Q member 2 (KCNQ2) by PRMT1 could positively regulate KCNQ activity by enhancing its binding to PIP2 to prevent neuronal hyperexcitability and seizures [ xref ]."
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"GST-KCNQ2 was methylated by Prmt1, but not by other Prmts (XREF_FIG and XREF_FIG)."
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"These results strongly advocate that Prmt1 interacts with and methylates KCNQ2."
27466704
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"Prmt1 interacts with and methylates KCNQ2."
27466704