IndraLab

Statements

USP18 binds IRS4. 44 / 44
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sparser
"Our results suggested that overexpressed IRS4 promoted while knockdown IRS4 inhibited the Jak/STAT signaling pathway by the interaction of IRS4 and USP18."
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reach
"And to detect whether IRS4 interacts with USP18 endogenously, immunoblot analysis of whole cell lysates and anti-IRS4 or lgG IP derived from Huh7.5.1 cells or 293T cells were performed."
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sparser
"Maybe the unique mechanism is the interaction with USP18 and IRS4."
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sparser
"IRS4 binding to USP18 diminished the inhibitory effect of USP18 on Jak/STAT signaling."
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sparser
"In addition, IRS4 (401–1257) bound to USP18 (Figure xref ), but not IRS4 region 401-1093 (Figure xref )."
29285303
sparser
"To determine which region of USP18 binds to IRS4, the Flag-tagged deletion mutants of USP18 was used for binding studies (Figure xref )."
29285303
sparser
"In contrast, we showed that USP18 C-terminal region (amino acid residue 321-372) bound to IRS4 (Figure xref )."
29285303
sparser
"Furthermore, Two IRS4 mutants (1-334, 401-1093), which did not interact with USP18, did not affect Jak/STAT signaling ( xref )."
29285303
sparser
"These results suggested that IRS4 binds to USP18 to diminish the blunting effect of USP18 on IFN-a-induced Jak/STAT signaling."
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reach
"IRS4 binding to USP18 diminished the inhibitory effect of USP18 on Jak and STAT signaling."
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reach
"These results suggested that IRS4 binds to USP18 to diminish the blunting effect of USP18 on IFN-a-induced Jak and STAT signaling."
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"And this IRS4 and USP18 interaction diminish USP18 's inhibitory effect on Jak and STAT signaling, therefore to potentiate the anti-HCV effect of IFN-a."
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"To confirm whether USP18 specifically binds to IRS4, immunoblot analysis of whole cell lysates and anti-FLAG M2 affinity IP derived from 293T cells after cotransfection with plasmids encoding Flag (empty vector) or Flag tagged USP18 and Myc tagged IRS4 were performed."
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sparser
"Furthermore, two mutants IRS4(1-334) and IRS4(401–1093) that do not bind to USP18 have no effect on interferon anti-HCV activity (Figure xref )."
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reach
"Co-IP assay demonstrated that USP18 binds to IRS4."
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reach
"In addition, IRS4 (401-1257) bound to USP18, but not IRS4 region 401-1093."
29285303
reach
"To examine whether over-expressed USP18 can interact with endogenous IRS4, Flag or Flag tagged USP18 were over-expressed in 293T cells and immunoprecipitated with an anti-Flag M2 affinity assay."
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reach
"Co-IP assay clearly demonstrated that USP18 interacts with endogenous IRS4."
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reach
"Lastly, to examine whether IRS4 interacts with USP18 endogenously in JFH1 infected cells, immunoblot analysis of whole cell lysates and anti-USP18 or lgG IP derived from JFH1 infected Huh7.5.1 cells were performed."
29285303
reach
"These results collectively demonstrated that IRS4 interacted with USP18 endogenously."
29285303
reach
"Co-IP assay demonstrated that IRS4 (1-400) bound to USP18."
29285303
reach
"Our results suggested that overexpressed IRS4 promoted while knockdown IRS4 inhibited the Jak and STAT signaling pathway by the interaction of IRS4 and USP18."
29285303
biogrid
No evidence text available
29285303
biogrid
No evidence text available
29285303
sparser
"Co-IP assay demonstrated that USP18 binds to IRS4 (Figure xref )."
29285303
reach
"In this study, combination of IP and MS screening and in vitro functional studies identified that IRS4 interacts with USP18 endogenously."
29285303
reach
"We also showed that USP18 binds to IRS4 primarily through the C-terminal region (amino acids 321-372) and thus to inhibit downstream Jak and STAT signal transduction."
29285303
reach
"In this study, we used JFH1 HCV culture model to dissect the role of IRS4 and USP18 interaction in IFN-a anti-HCV activity."
29285303
reach
"To determine which region of USP18 binds to IRS4, the Flag tagged deletion mutants of USP18 was used for binding studies."
29285303
reach
"In contrast, we showed that USP18 C-terminal region (amino acid residue 321-372) bound to IRS4."
29285303
sparser
"In this study, we showed that IRS4 binds to the C-terminus of USP18."
29285303
sparser
"To confirm whether USP18 specifically binds to IRS4, immunoblot analysis of whole cell lysates and anti-FLAG M2 affinity IP derived from 293T cells after cotransfection with plasmids encoding Flag (empty vector) or Flag-tagged USP18 and Myc-tagged IRS4 were performed."
29285303
sparser
"And to detect whether IRS4 interacts with USP18 endogenously, immunoblot analysis of whole cell lysates and anti-IRS4 or lgG IP derived from Huh7.5.1 cells or 293T cells were performed."
29285303
sparser
"Lastly, to examine whether IRS4 interacts with USP18 endogenously in JFH1-infected cells, immunoblot analysis of whole cell lysates and anti-USP18 or lgG IP derived from JFH1-infected Huh7.5.1 cells were performed."
29285303
sparser
"These results collectively demonstrated that IRS4 interacted with USP18 endogenously."
29285303
sparser
"Co-IP assay demonstrated that IRS4 (1–400) bound to USP18 (Figure xref )."
29285303
sparser
"However, IRS4(1–334) did not bind to USP18 (Figure xref )."
29285303
biogrid
No evidence text available
29285303
sparser
"To examine whether over-expressed USP18 can interact with endogenous IRS4, Flag or Flag-tagged USP18 were over-expressed in 293T cells and immunoprecipitated with an anti-Flag M2 affinity assay."
29285303
sparser
"Co-IP assay clearly demonstrated that USP18 interacts with endogenous IRS4 (Figure xref )."
29285303
sparser
"Similarly, over-expressed exogenous IRS4 was also able to interact with endogenous USP18 (Figure xref )."
29285303
sparser
"In this study, combination of IP and MS screening and in vitro functional studies identified that IRS4 interacts with USP18 endogenously."
29285303
sparser
"We also showed that USP18 binds to IRS4 primarily through the C-terminal region (amino acids 321-372) and thus to inhibit downstream Jak/STAT signal transduction."
29285303
sparser
"We identified that amino acids 335–400 and amino acids 1094-1257 of IRS4 are important for the IRS4-USP18 interaction."
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