IndraLab

Statements

OTUB1 increases the amount of CTNNB1. 9 / 9
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"OTUB1, known as a K48 linkage-specific deubiquitinating enzyme, promotes β-catenin expression by suppressing its protein degradation and regulates CRC metastasis [91]."
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"To exclude the effects of transcriptional regulation, we conducted RT-PCR and found that decreased OTUB1 failed to inhibit β-catenin mRNA expression (Fig. 4H)."
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"The results further showed that OTUB1 restrains significantly the ubiquitination of β-catenin; while OTUB1 C91S cannot; decreased OTUB1 promotes the ubiquitination level of β-catenin (Fig. 5I)."
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"In contrast, the knockdown of OTUB1 in 11Z and HESC cells increased the expression of E-cadherin and decreased the expression of Vimentin, β-catenin, and α-SMA at the mRNA level (Figure 5F)."
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"The above results implied that OTUB1 promotes β-catenin expression and stability at the protein level instead of the mRNA level."
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"The fact that OTUB1 binds with β-catenin has been identified, and the results of immunofluorescence showed that elevated OTUB1 promotes β-catenin expression and its nuclear import, which further regulates the complicated transcription processes (Fig. 5A)."
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"The results showed that the expression of β-catenin and its downstream targets (AXIN-2, C-myc, cyclin D1 and TCF1) were induced by OTUB1 overexpression, which was further restrained as the concentration of XAV-939 gradually increased, OTUB1 expression has no significant changes (Fig. 6A)."
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"The results showed that the expression of β-catenin and its targets was increased by elevated OTUB1 and β-catenin, while increased expression was further restrained by XAV-939 in the T24 cell (Fig. 7H)."
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"Furthermore, we further found that the expression of β-catenin, ki-67 and downstream targets are induced by overexpressed OTUB1, which is conversely inhibited by XAV-939."
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