IndraLab

Statements


SPINT1 dephosphorylates OTUD4. 5 / 5
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sparser
"Second, HIN1HAI1 interaction, as well as HAI1 dephosphorylation of HIN1, provides a link between stress signaling and pre-mRNA splicing."

reach
"To determine whether HAI1 could dephosphorylate HIN1, YFP-HIN1 was immunoprecipitated, incubated with recombinant HAI1 or nonspecific phosphatase (C.I.P."

reach
"In contrast, HAI1 could not fully dephosphorylate the N-terminal HIN1 truncation (bands 3 and 4 on Phostag gel and band 5 on SDS/PAGE; Fig. 1E)."

reach
"However, it is unlikely that this occurs via effects on HIN1 RNA binding since this occurred at the N-terminal region, while HAI1 interacted with and dephosphorylated the C-terminal portion of HIN1."

sparser
"To determine whether HAI1 could dephosphorylate HIN1, YFP-HIN1 was immunoprecipitated, incubated with recombinant HAI1 or nonspecific phosphatase (C.I.P.), and analyzed on Phos-Tag gels."