IndraLab

Statements


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"We present that the first interacting molecule, β-COP, directly interacting with N-terminal region of TREK1."

sparser
"Β-COP binds directly to TREK1 but not TWIK1 in a heterologous expression system."

sparser
"In this study, we provide an evidence for a direct protein–protein interaction between β-COP, a subunit of Coat Protein Complex I (COPI), and the TREK1 channel."

sparser
"This protein–protein interaction between β-COP and TREK1 channel was confirmed by several in vitro assays and in vivo assays ( Figs."

sparser
"The direct binding of β-COP with TREK1 in vivo was strongly supported by Immunoprecipitation experiment with an anti-TREK1 antibody in PC3 cells ( Fig. 2 C) and by the co-localization of RFP-β-COP w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In this study, we demonstrated for the first time that β-COP directly interacts with N-terminal region of TREK1."

sparser
"To investigate the interaction between TREK1 and β-COP in vivo , N-terminal tagged vectors expressing Flag-tagged TREK1 (Flag-TREK1) and GFP-tagged β-COP (GFP-β-COP) were co-transfected into HEK293T [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Especially, we clearly showed that β-COP was associated with TREK1 in native condition at the PC3 cells ( Fig. 2 C)."

sparser
"The protein–protein interaction between TREK1 and β-COP was confirmed using the yeast two-hybrid and GST pull-down assays."

sparser
"Based on these data, we concluded that β-COP can directly bind with the N-terminal region of TREK1 channels."

sparser
"Using the yeast two-hybrid technique of the human cDNA library on the N-terminal region of TREK-1, a direct β-COP-TREK-1 interaction was reported ( xref )."