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COPB1 binds KCNK2. 32 / 32
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"In this study, we demonstrated for the first time that β-COP directly interacts with N-terminal region of TREK1."
20362547
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"Therefore, TREK1 binds to β-COP near the cell membrane, TREK2 binds to β-COP near the nucleus in a condensed state, and TRAAK does not bind to β-COP."
37296621
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"Β-COP Is Associated with Endogenous TREK1 in Cultured Astrocytes."
36291187
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"We also performed bimolecular fluorescence complementation (BiFC) to determine the interactions between TREK1 isoforms and β-COP."
36291187
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"Based on these data, we concluded that β-COP can directly bind with the N-terminal region of TREK1 channels."
20362547
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"Β-COP binds to TREK1 at the N-terminus to increase membrane ex-pression, whereas it binds to the C-terminus of TREK2 to decrease membrane expression, and β-COP does not associate with TRAAK."
37296621
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"The protein–protein interaction between TREK1 and β-COP was confirmed using the yeast two-hybrid and GST pull-down assays."
20362547
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"To investigate the interaction between TREK1 and β-COP in vivo , N-terminal tagged vectors expressing Flag-tagged TREK1 (Flag-TREK1) and GFP-tagged β-COP (GFP-β-COP) were co-transfected into HEK293T [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20362547
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"The interaction between the TREK1 N-terminus deletion forms and β-COP was investigated using co-IP ( xref B)."
36291187
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"Therefore, we predicted that β-COP interacts with the N4 region of TREK1, and thus produced a vector in which only the N4 region was deleted from the N-terminus of TREK1 (TREK1NΔ4) ( xref A)."
36291187
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"To test whether β-COP binds to TREK1 by recognizing the KXKXX sequence at the end of the N-terminal region of TREK1, we constructed an AWA mutant of TREK1 by substituting AXA for KXK in TREK1 using mutagenesis cloning."
36291187
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"Since β-COP interacts with TREK1 and regulates its surface expression in astrocytes, we next tested whether β-COP also interacts with TWIK1."
36291187
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"Using the yeast two-hybrid technique of the human cDNA library on the N-terminal region of TREK-1, a direct β-COP-TREK-1 interaction was reported ( xref )."
31031627
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"Previous studies have indicated that β-COP binds to the N-terminus of TREK1 and increases its surface expression [ xref ]."
37296621
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"In our previous study, we reported that the interaction between β-COP and TREK1 led to an increase in the surface expression of TREK1 [ xref ]."
37296621
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"As a result, TWIK1 could not bind to β-COP, whereas TREK1 strongly bound to β-COP ( xref C)."
36291187
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"Unlike TREK1, which binds to β-COP at the N-terminal motif, TREK2 binds to β-COP in the TREK2ΔN ( xref B)."
37296621
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"Building on this finding, in the present study, we aimed to investigate the interaction of β-COP with other members of the TREK subfamily."
37296621
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"This study was conducted based on previously reported experimental results that β-COP directly binds to the N-terminus of TREK1 [ xref ]."
36291187
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"However, our current results show that β-COP binds to TREK1 and enhances the surface expression and channel activity of TREK1 in astrocytes without the involvement of other coatomers of the COP1 complex to control surface expression of the channel ( xref , xref and xref )."
36291187
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"Therefore, we compared the interactions between the two TREK1 isoforms and β-COP, although no difference was detected ( xref )."
36291187
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"In this study, we provide an evidence for a direct protein–protein interaction between β-COP, a subunit of Coat Protein Complex I (COPI), and the TREK1 channel."
20362547
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"Using the deleted TREK1 mutants, we showed that β-COP binds to the di-lysine (KXKXX) motif at the end of the TREK1 N-terminus."
36291187
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"A strong signal was observed for the binding of TREK1 to β-COP, as in a previous study [ xref ], but no signal was observed for TWIK1( xref )."
36291187
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"While β-COP binds to the N-terminus of TREK1 [ xref ], it binds to the RRR motif in the C2 region of TREK2."
37296621
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"Β-COP binds directly to TREK1 but not TWIK1 in a heterologous expression system."
36291187
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"Previously, we reported that β-COP binds directly to TREK1 in a heterologous expression system [ xref ]."
36291187
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"The direct binding of β-COP with TREK1 in vivo was strongly supported by Immunoprecipitation experiment with an anti-TREK1 antibody in PC3 cells ( Fig. 2 C) and by the co-localization of RFP-β-COP w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20362547
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"This protein–protein interaction between β-COP and TREK1 channel was confirmed by several in vitro assays and in vivo assays ( Figs."
20362547
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"Especially, we clearly showed that β-COP was associated with TREK1 in native condition at the PC3 cells ( Fig. 2 C)."
20362547
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"We present that the first interacting molecule, β-COP, directly interacting with N-terminal region of TREK1."
20362547
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"In our previous study, we reported that β-COP binds directly to the N-terminal region of TREK1 [ xref ]."
36291187