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LPA activates KCNK18. 7 / 7
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"Upon co-expression in Xenopus oocytes LPA simultaneously increased both depolarising TRPV1 and hyperpolarising TRESK currents."
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"Increasing LPA concentrations augmented TRESK current amplitude with an EC of 0.2 μM (Fig. 2C)."
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"To confirm a Gαq-coupled signalling pathway of LPA-induced augmentation of TRESK currents we used the specific blocker of phospholipase C (PLC) U73122."
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"Pre-incubation of oocytes in a solution containing U73122 significantly reduced LPA-mediated TRESK augmentation to 0.4 ± 0.12 fold (n = 6, two-tailed unpaired t-test, p = 0.006; Fig. 2D, middle trace)."
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"Interestingly, LPA can activate TWIK‐related spinal cord potassium channel (TRESK) receptors in mouse DRG neurons, which decreases neuronal firing and potentially counteracts its effect on TRPV1 (Kollert et al. 2015)."
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"As TRESK channels and TRPV1 receptors are both activated by LPA we next investigated LPA induced effects in the same oocyte co-injected with TRPV1 and TRESK cRNA."
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"Both channels account for significant current components in DRG neurons5722.In the present study we use heterologous gene expression to show that LPA strongly activates TRESK channels by its Gαq-coupled receptors."
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