IndraLab

Statements

CALM1 binds UMOD. 20 / 20
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"The selective expression of CXCL10 , CXCL11 , and IL1-β by THP-CAM validates their use as a model for CAM."
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"Upon stimulation with LPS, THP-CAM preferentially expressed IFN-β and IFN-λ1, MyD88, STAT1 and the ISGs: IFIT1, Mx1, CXCL10, OAS1 and ISG15."
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"Unlike the primary human macrophages, however, THP-CAM did not express IFN-α1, the lowest affinity IFN-α subtype."
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"Since IFN-α1 is expressed by primary macrophages in response to synthetic ligands and virulent Mycobacterium tuberculosis (MTB), THP-CAM may provide an opportunity to explore the function of this uniq[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"As shown in Fig. 1 B, THP-CAM and -AAM are adherent with typical flat, elongated and branching macrophage morphology."
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"To validate the THP-CAM and -AAM experimental models, we measured the expression of a representative set of pro- and anti-inflammatory genes using qRT-PCR."
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"After polarization ( Fig. 2 A), THP-CAM preferentially expressed pro-inflammatory genes encoding CXCL10 and CXCL11 , whereas THP-AAM preferentially expressed anti-inflammatory genes encoding the manno[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"After stimulating the polarized cells with LPS ( Fig. 2 B), THP-CAM preferentially secreted IFN-γ and IL-1β, whereas THP-AAM preferentially secreted IL-10 and IL-4 into the supernatant."
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"Indeed, Fig. 3 shows that while STAT6 is expressed equally in both THP-CAM and -AAM, it is only activated in THP-AAM."
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"Having demonstrated that THP-1 monocytes can be polarized to express CAM- or AAM-oriented markers, we used the THP-CAM and -AAM models to investigate differential expression of a panel of 84 IFN α/β p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"As shown in Fig. 4 , a subset of key ISGs (e.g., Mx1, ISG15, etc.) were preferentially expressed by THP-CAM while their expression in THP-AAM was markedly impaired at both time points."
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"Having demonstrated that THP-CAM and -AAM differentially expressed IFN α/β pathway genes, we then used a sensitive and specific qRT-PCR assay developed in our laboratory to measure expression of the 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Analysis of types I and III IFN expression in THP-CAM and -AAM after 1 and 4 h of LPS stimulation revealed that out of the 16 genes we measured, 5 were consistently expressed in THP-CAM and -AAM: IFN-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Specifically, IFN-β and -λ1 were expressed after 4 h of LPS stimulation only in THP-CAM ( Fig. 5 , top)."
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"Both THP-CAM and -AAM expressed IFN-α subtypes and there was a trend towards weighted expression by the THP-CAM of IFN-α4 and -α10 ( Fig. 5 , bottom)."
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"We then sought to demonstrate that the patterns of expression of CAM and AAM-related markers and IFN-β and -λ1 genes in the THP-CAM and -AAM model reflect expression in primary human monocyte derived [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Similar to THP-CAM, IFN-β and -λ1 were expressed after 4 h of LPS stimulation only in MD-CAM ( Fig. 7 , top)."
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"For example, THP-AAM preferentially express MRC1 , the gene for the mannose receptor, and CCL22 , while THP-CAM preferentially express CXCL10 and CXCL11 ."
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"Upon LPS stimulation, THP-AAM preferentially express IL-4 and IL-10, while THP-CAM preferentially express IL-1β and IFN-γ."
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"In addition, THP-AAM show qualitative, quantitative, and kinetic differences in the expression of types I and III IFN and IFN α/β pathway genes compared to THP-CAM that extend to their primary human m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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