IndraLab

Statements

SMAD1 binds UCHL3. 17 / 18
2 | 1 14
sparser
"It has been reported that UCHL3 can interact with SMAD1, a classical osteogenesis-related molecule involved in BMSC osteogenesis [ xref , xref , xref ]."
36915132
sparser
"The protein‒protein docking results from Alphafold 2 revealed that UCHL3 could directly bind with SMAD1 through hydrogen bonds (Fig.  xref i)."
36915132
sparser
"Further immunofluorescence and co-immunoprecipitation (Co-IP) verified the interaction between UCHL3 and SMAD1 in BMSCs (Fig.  xref j–l)."
36915132
sparser
"Consistently, our data showed that UCHL3 physically interacted with SMAD1 and that its expression in BMSCs was positively correlated with SMAD1."
36915132
sparser
"To investigate the interaction between Uch-L3 and Smad1, HA-Smad1 and Flag-Uch-L3 plasmids were transfected into HEK 293 cells."
21453705
sparser
"Furthermore, it wondered whether the endogenous Smad1 interacts with Uch-L3."
21453705
sparser
"Furthermore, the endogenous Uch-L3 was also interacted with the endogenous Smad1 in C2C12 cells as confirmed by co-IP against anti-Uch-L3 antibody and WB using anti-Smad1 antibody ( Fig. 1 E)."
21453705
sparser
"All of these results showed that Uch-L3 interacts with Smad1."
21453705
sparser
"After confirming the interaction between Smad1 and Uch-L3, we tested whether the deubiquitinating activity of Uch-L3 is able to affect the ubiquitination pattern of Smad1."
21453705
sparser
"UCH-L3 physically interacts with Smad1 and stabilizes it by deubiquitylating its polyubiquitin."
30123285
biogrid
No evidence text available
21453705
sparser
"The physical interaction between Uch-L3 and Smad1 was revealed."
21453705
sparser
"For example, UCHL3 physically interacts with Smad1 and decreases its poly-ubiquitylation ( xref )."
29898404
biogrid
No evidence text available
21453705
sparser
"In this study, it was found Smad1 is physically interacted with Uch-L3, which deubiquitinates Smad1."
21453705
reach
"For example, UCHL3 physically interacts with Smad1 and decreases its poly-ubiquitylation."
29898404
sparser
"At the mechanistic level, we showed that SMAD1 interacted with UCHL3 in BMSCs and was downregulated when UCHL3 was suppressed."
36915132