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UCHL3 increases the amount of POLD4. 3 / 3
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"Indeed, we found that silencing UCHL3 reduced POLD4 protein expression (not mRNA), which was reversed by the UPS inhibitor MG132 (Fig. 5F and Fig. S6K)."
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"Overexpression of wild-type UCHL3 (WT-UCHL3), but not the catalytically dead mutant S75A, induced elevated POLD4 levels in a dose-dependent manner in HEK293T cells (Fig. 5H), implying that the enzyme activity of UCHL3 was crucial for stable POLD4."
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"Stabilization of POLD4 assays revealed that ectopic expression of K48R (not WT) ubiquitin restored the POLD4 expression inhibited by UCHL3 depletion in MES GSCs (Fig. 5M)."
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