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USP22 binds Flag. 9 / 9
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"To investigate the effect of USP22 overexpression on hepatocellular carcinoma cell proliferation, we infected HepG2 and Hep3B cells with lentiviruses and established cell lines stably overexpressing Flag‐USP22 or not (Figure xref )."
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"We found that transduction efficiencies were more than 95% and comparable between Jurkat (Flag) and Jurkat (Flag-USP22) cells ( Fig. 4 C)."
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"The lysates of HEK‐293FT cells expressing Flag‐USP22 were prepared and subjected to Flag affinity purification."
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"Total proteins from HEK‐293FT cells expressing Flag‐USP22 or control vectors were first immunoprecipitated with anti‐Flag, followed by immunoblotting with antibodies against CDK11B. The results showed that Flag‐USP22 indeed interacted with CDK11B (Figure  xref )."
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"USP22 protein contains an N‐terminal zinc‐finger (ZnF) and a C19 ubiquitin‐specific peptidase (C19 peptidase) domain. xref , xref To illustrate the molecular detail involved, co‐IP assays were performed in HEK‐293FT cells expressing Flag (vector), Flag‐USP22, Flag‐USP22 (1‒160 aa) or Flag‐USP22 (161‒525 aa) using anti‐Flag."
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"Similar results were obtained in the exogenous Co-IP assays that were carried out on HEK293T cells transfected with Flag-USP22 or/and HA-c-Myc ( Figures 5 C and D), which corroborated that c-Myc is a [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Cells were transfected with HA-c-Myc combined with or without Flag-USP22 for 48 hours."
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"The plasmids encoding Flag‐USP22 wild‐type or catalytically inactive Flag‐USP22 HH/AA (Figure  xref ) were co‐transfected with HA‐ubiquitin into HEK‐293FT cells."
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"The endogenous NFATc2 protein level was higher in Jurkat (Flag-USP22) cells and Jurkat (Flag) cells treated with MG132 than Jurkat (Flag) cells ( Fig. 4 H)."
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