IndraLab

Statements

USP19 is palmitoylated. 14 / 14
| 14
sparser
"Then, we found that ZDHHC2 decreases the lipid peroxide production and suppresses ferroptosis through mediating the S‐palmitoylation of USP19 and destabilizing ACSL4 in CRPC cells."
41126755
sparser
"To confirm whether ZDHHC2 is the only ZDHHC enzyme that mediates the palmitoylation of USP19, a set of ZDHHC‐knockout cell lines was established using a CRISPR screen."
41126755
sparser
"We discovered that USP19 inhibits the ubiquitin–proteasome pathway‐mediated degradation of ACSL4 and that ZDHHC2 enhances ACSL4 degradation by palmitoylating USP19, ultimately suppressing ferroptosis."
41126755
sparser
"ZDHHC2 Mediates the Palmitoylation of USP19."
41126755
sparser
"Therefore, we propose that USP19 palmitoylation attenuates its interaction with ACSL4, thereby facilitating ACSL4 ubiquitination and subsequent degradation."
41126755
sparser
"Under enzalutamide resistance, the FOXA1/CXXC5/TET2 complex induces aberrant upregulation of ZDHHC2, which subsequently suppresses ferroptosis by mediating S‐palmitoylation of USP19 and degradation of ACSL4 protein in CRPC cells."
41126755
sparser
"We found that palmitic acid elevated palmitoylation of USP19 (Figure  xref , Supporting Information) and reduced ACSL4 protein level (Figure  xref , Supporting Information), and these effects disappeared after ZDHHC2 knockout (Figure  xref , Supporting Information)."
41126755
sparser
"ZDHHC2 is transcriptionally upregulated by a FOXA1/CXXC5/TET2 complex and promotes S‐palmitoylation of the deubiquitinase USP19, which impairs its interaction with ACSL4."
41126755
sparser
"In the resistant cell lines, mRNA level of USP19 and ACSL4 remained unchanged (Figure  xref , Supporting Information), palmitoylation of USP19 was elevated, and ACSL4 protein was decreased (Figure  xref , Supporting Information)."
41126755
sparser
"Only ZDHHC2 knockout significantly abolished the palmitoylation of USP19 (Figure  xref ; Figure  xref , Supporting Information)."
41126755
sparser
"The Palmitoylation of USP19 Weakens its Interaction with ACSL4 and Promotes Enzalutamide Resistance by Inhibiting Ferroptosis."
41126755
sparser
"To explore the effect of USP19 palmitoylation, we assessed the protein level of USP19 following ZDHHC2 knockdown; however, no change was observed. (Figure  xref , Supporting Information)."
41126755
sparser
"Next, we explored whether ZDHHC2 regulates ACSL4 levels by palmitoylating USP19."
41126755
sparser
"Additionally, the increase in ACSL4 protein caused by USP19 C152S mutant overexpression could not be reversed by ZDHHC2 overexpression (Figure  xref ), which further confirmed that ZDHHC2 promotes ACSL4 degradation by palmitoylating USP19."
41126755