IndraLab

Statements

UCHL3 activates RAD51. 6 / 6
3 | 3
signor
"Here we report that ubiquitination of RAD51 hinders RAD51-BRCA2 interaction, while deubiquitination of RAD51 facilitates RAD51-BRCA2 binding and RAD51 recruitment and thus is critical for proper HR. | UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2.|Our results suggested that three lysine sites (56, 57, and 63) on RAD51 that are close to E59 are deubiquitinated by UCHL3."
27941124
reach
"Re-expression of wild-type UCHL3 restored RAD51 IRIF, while expression of the S75A mutant did not (Fig. 5D; Supplemental Fig. S9B)."
27941124
signor
"Here we report that ubiquitination of RAD51 hinders RAD51-BRCA2 interaction, while deubiquitination of RAD51 facilitates RAD51-BRCA2 binding and RAD51 recruitment and thus is critical for proper HR. | UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2.|Our results suggested that three lysine sites (56, 57, and 63) on RAD51 that are close to E59 are deubiquitinated by UCHL3."
27941124
reach
"However, overexpression of UCHL3-mediated resistance to radiation and PARPi was abolished in RAD51-depleted cells (Fig. 6G,H)."
27941124
reach
"Another complimentary possibility is that, unlike BRCA2/RAD51 knockout, UCHL3 knockout only decreases Rad51 function."
27941124
signor
"Here we report that ubiquitination of RAD51 hinders RAD51-BRCA2 interaction, while deubiquitination of RAD51 facilitates RAD51-BRCA2 binding and RAD51 recruitment and thus is critical for proper HR. | UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2.|Our results suggested that three lysine sites (56, 57, and 63) on RAD51 that are close to E59 are deubiquitinated by UCHL3."
27941124