IndraLab

Statements

UCHL3 activates RAD51. 6 / 6
3 | 3
reach
"Another complimentary possibility is that, unlike BRCA2/RAD51 knockout, UCHL3 knockout only decreases Rad51 function."
27941124
signor
"Here we report that ubiquitination of RAD51 hinders RAD51-BRCA2 interaction, while deubiquitination of RAD51 facilitates RAD51-BRCA2 binding and RAD51 recruitment and thus is critical for proper HR. | UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2.|Our results suggested that three lysine sites (56, 57, and 63) on RAD51 that are close to E59 are deubiquitinated by UCHL3."
27941124
signor
"Here we report that ubiquitination of RAD51 hinders RAD51-BRCA2 interaction, while deubiquitination of RAD51 facilitates RAD51-BRCA2 binding and RAD51 recruitment and thus is critical for proper HR. | UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2.|Our results suggested that three lysine sites (56, 57, and 63) on RAD51 that are close to E59 are deubiquitinated by UCHL3."
27941124
reach
"Re-expression of wild-type UCHL3 restored RAD51 IRIF, while expression of the S75A mutant did not (Fig. 5D; Supplemental Fig. S9B)."
27941124
signor
"Here we report that ubiquitination of RAD51 hinders RAD51-BRCA2 interaction, while deubiquitination of RAD51 facilitates RAD51-BRCA2 binding and RAD51 recruitment and thus is critical for proper HR. | UCHL3, in turn, deubiquitinates RAD51 and promotes the binding between RAD51 and BRCA2.|Our results suggested that three lysine sites (56, 57, and 63) on RAD51 that are close to E59 are deubiquitinated by UCHL3."
27941124
reach
"However, overexpression of UCHL3-mediated resistance to radiation and PARPi was abolished in RAD51-depleted cells (Fig. 6G,H)."
27941124