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Statements

YOD1 binds MYC. 11 / 11
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"To investigate whether the α-synuclein protein stability is mediated by YOD1, Myc-YOD1 was transfected in a dose-dependent manner."
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"In this study, we investigated the ubiquitination of Myc-YOD1 and Flag-USP21 using wild-type HA- Ub and various lysine mutant forms of it."
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"To evaluate the effect of YOD1 on the stability of USP21, we transfected HEK293T, HeLa, and A549 cell lines with increasing doses of Myc-YOD1 and assessed the expression level of exogenous USP21."
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"Additionally, we examined which lysine-linked polyubiquitin chain is associated with the proteasomal degradation of Myc-YOD1."
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"Subsequently, we transfected increasing doses of Flag- USP21 into HEK293T, HeLa, and A549 cells to evaluate the stability change of Myc-YOD1."
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"Surprisingly, each lysine mutant form of HA-Ub was capable of forming polyubiquitin chains on Myc-YOD1, and each type of lysine mutant form-linked polyubiquitin chain on Myc-YOD1 was related to proteasomal degradation (Fig.  xref C)."
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"Interestingly, we found that the expression level of Myc-YOD1 remained unchanged in HEK293T cells exposed to Flag- USP21 (Fig.  xref C), and there was no significant difference in YOD1 stability observed in HeLa and A549 cells (Fig.  xref D)."
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"For deubiquitination assay, Flag-α-synuclein, HA-ubiquitin-WT, its lysine mutants (HA-Ub + K6 , HA-Ub + K11 , HA-Ub + K27 , HA-Ub + K29 , HA-Ub + K33 , HA-Ub + K48 , and HA-Ub + K63 ), and Myc-YOD1 we[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We observed that the expression level of NEDD4 was significantly increased, and α-synuclein was decreased in a dose-dependent manner of Myc-YOD1 ( Fig. 4 A and B)."
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"In the deubiquitination assay of Myc-YOD1, the formation of a polyubiquitin chain on Myc-YOD1 was reduced by Flag-USP21 compared to a catalytically inactive mutant of Flag-USP21 (C221S), indicating that USP21 functions as a DUB of YOD1 (Fig.  xref A)."
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"Similarly, in the deubiquitination assay of Flag-USP21, the formation of a polyubiquitin chain on Flag-USP21 was reduced by Myc-YOD1 compared to a catalytically inactive mutant of Myc-YOD1 (C160S), indicating that YOD1 functions as a DUB of USP21 (Fig.  xref B)."
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