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"In the indicated experiments, the conditional overexpression of Usp13 in hepatocytes (Hep OE) were triggered by injection of adeno-associated virus serotype-8 (AAV8)-thyroxine-binding globulin promoter (TBG)-recombinase Cre vector (AAV8-TBG-Cre) via intravenous injection and then determined by immunoblotting assay."