IndraLab

Statements

COCH binds KCNK2. 26 / 27
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"To assess if cochlin interaction with TREK-1 also modulates the channel current, human TREK-1 was transiently expressed in HEK293 cells, and channel activity was monitored using whole-cell patch clamping."
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"Voltage sensitive dye, bis-(1, 3-dibutylbarbituric acid) trimethine oxonol (DiBAC) intake studies XREF_BIBR further showed that TREK-1 interaction with cochlin is functional resulting in activation of TREK-1 channel and concomitant changes in TM cell shapes XREF_BIBR."
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"These observations are consistent with and corroborates our previous studies xref , xref , demonstrating similar changes in cell shape and interaction of multimerized cochlin with TREK-1 channels xref ."
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"Previously, we postulated a model in which multimerized cochlin binding to TREK-1 changes cell shape and motility."
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"A direct or functional interaction of cochlin and TREK-1 remains to be demonstrated, however, a potential functional interaction between cochlin and TREK-1 may exist XREF_BIBR."
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"This data supports the idea that monomeric cochlin interacts with TREK-1 in the physiological environment causing a small increase in TREK-1 current resulting in a positive effect on outflow and a reduction in IOP."
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"Previously, we postulated a model in which multimerized cochlin binding to TREK-1 changes cell shape and motility."
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"To investigate if the cochlin-TREK-1 interaction produced significant rearrangements in cellular organization within the TM cells, we measured fluorescein dye transport using an Ussing-type chamber across a trilayer of TM cells cultured on a PVDF membrane (Fig.  xref )."
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"Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure."
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"Results presented here indicate that the interaction of TREK-1 and cochlin play an important role for maintaining IOP homeostasis. ""
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"We next probed functional interaction between cochlin and TREK-1 using the voltage sensitive dye, bis-(1, 3-dibutylbarbituric acid) trimethine oxonol (DiBAC)."
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"Taken together, these data suggest interaction of cochlin with TREK-1 is involved in formation of filopodia-like protrusions."
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"A direct or functional interaction of cochlin and TREK-1 remains to be demonstrated, however, a potential functional interaction between cochlin and TREK-1 may exist xref ."
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"The TREK-1 and cochlin interaction is not sufficiently supported with the collagen gel assays and fluorescein transport assays (Fig.  xref ) alone but they lay the foundation for the remainder of our studies."
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"The data presented here suggests that mechanical forces are possibly transmitted through cochlin-TREK-1 protein-protein interactions."
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"Cochlin-TREK-1 interaction has functional consequences and results in changes in cell shape and motility."
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"This data supports the idea that monomeric cochlin interacts with TREK-1 in the physiological environment causing a small increase in TREK-1 current resulting in a positive effect on outflow and a reduction in IOP."
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"The interaction of cochlin and TREK-1 becomes an area of interest as both are components that seem to be needed for IOP regulation, as seen in the silencing experiments."
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"This is consistent with the observation that similar amounts of cochlin are associated with greater amounts of TREK-1 in human TM samples, modeled through the application of shear stress (Fig.  xref )."
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"The TREK-1 and cochlin interaction is not sufficiently supported with the collagen gel assays and fluorescein transport assays alone but they lay the foundation for the remainder of our studies."
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"These results suggest basement membrane proteins are elevated concomitant with secretion of cochlin in glaucomatous tissue and interaction of cochlin with TREK-1, stretch-activated channel (SAC) proteins and annexin A2 may mediate effects of cochlin."
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"Results presented here indicate that the interaction of TREK-1 and cochlin play an important role for maintaining IOP homeostasis."
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"The interaction of cochlin and TREK-1 becomes an area of interest as both are components that seem to be needed for IOP regulation, as seen in the silencing experiments."
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"To assess if cochlin interaction with TREK-1 also modulates the channel current, human TREK-1 was transiently expressed in HEK293 cells, and channel activity was monitored using whole-cell patch clamping."
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"Cochlin interacts with Annexin A2, SLC44A2, and potentially TREK-1."
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"Our data, for the first time, suggests the involvement of an interaction of cochlin and TREK-1 in glaucoma and renders this interaction a target for therapy."
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