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CDKN1A binds CALM1. 22 / 22
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"Genomic DNA from CAM-P21 was extracted using the High Pure viral nucleic acid kit (Roche, Mannheim, Germany)."
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"The genomic DNA of phage CAM-P21 was completely sequenced with an Illumina HiSeq 4000 platform using the 2 × 150-bp paired-end strategy."
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"The three phages (CAM-P21, PC10, and STG2) used in this study were listed in Table 1 ."
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"Phage CAM-P21, a member of Siphoviridae family, effective against C. coli, was kindly provided by the Fukuoka City Institute of Health and Environment, Fukuoka."
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"CAM-P21 has a linear double-stranded DNA (dsDNA) genome 12,587 bp long with an average GC content of 31.19%."
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"Genome of CAM-P21 (GenBank accession number MW462221) does not contain any genes associated to antibiotic resistance, toxins, and virulent factors ( Huang et al., 2021 )."
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"No genes related to virulence or resistance were observed according to the genome of CAM-P21, PC10 and STG2."
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"In the case of phage CAM-P21, which is specific to C. coli , C. coli CAN10 cells were harvested from 15 mL of the culture by centrifugation at 6500× g for 5 min at 25 °C and resuspended in 1 mL of BHI[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To 4.8 mL of the cell suspension, 0.1 mL of 5 × 10 8 PFU/mL phage PC10 suspension and 0.1 mL of EDTA solution were added to attain a final concentration of 0.67 mM (0.025%) according to the concentrat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In the case of C. coli, to 4.8 mL of BHI broth containing the bacteria at 3 × 10 4 CFU/mL was mixed with 0.1 mL of phage CAM-P21 (1.5 × 10 8 PFU/mL), and the EDTA solutions (0.025%, 0.05%) to attain M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In the case of C. coli, 4.8 mL of BHI broth containing the bacterium at 3 × 10 4 CFU/mL was mixed with 0.1 mL of phage CAM-P21 suspension (1.5 × 10 9 PFU/mL) and 0.1 mL of EDTA solution to attain a fi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In the presence of phage CAM-P21 (MOI = 1), viable counts of C. coli increased by 2 log for 8 h and decreased by 1.3 log at 12 h."
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"However, xref characterized and described a rare member of the Siphoviridae family, CAM-P21, isolated from the beef grind."
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"CAM-P21 was described to possess a broad host range, a better titer, and enhanced performance under diverse stress conditions compared to the Myoviridae family of phages."
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"CAM-P21 reduced viable C. coli counts by more than two logs after a 12–24 h incubation period at both 42 and 37°C in vitro , respectively ( xref )."
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"Genes associated with antibiotic resistance, toxins, and virulence factors were not found in the genome of CAM-P21."
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"The combined use of phage CAM-P21 and 0.67 mM EDTA slightly inhibited the bacterial re-growth ( Fig. 4 A)."
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"C. coli was incubated in the presence of phage CAM-P21 (MOI = 10 3 ) in the presence and absence of 0.67 mM or 1.3 mM EDTA in BHI broth at 8 °C for 48 h, followed by the incubation at 42 °C for 48 h ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In the control and the presence of EDTA (0.67 mM or 1.3 mM), the viable counts of C. coli only slightly decreased after 48 h incubation at 8 °C. The viable counts in the presence of 0 (control), 0.67,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Bacteriophage CAM-P21, isolated from a beef mince sample in Japan using Campylobacter coli , has a 12,587-bp genome encoding 18 putative coding sequences with an average GC content of 31.19%."
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"Phage CAM-P21 was isolated from a beef mince sample collected in Fukuoka, Japan, using the double-layer agar method ( xref ) with C. coli CAN10 as the host strain (Fukuoka City Institute of Health and Environment), grown at 42°C in brain heart infusion (BHI) broth supplemented with 10 mM CaCl 2 under microaerobic conditions."
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"The fully sequenced genome of phage CAM-P21 has been deposited in the GenBank database under the accession number."
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