IndraLab

Statements


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"However, xref characterized and described a rare member of the Siphoviridae family, CAM-P21, isolated from the beef grind."

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"The fully sequenced genome of phage CAM-P21 has been deposited in the GenBank database under the accession number."

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"CAM-P21 reduced viable C. coli counts by more than two logs after a 12–24 h incubation period at both 42 and 37°C in vitro , respectively ( xref )."

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"Bacteriophage CAM-P21, isolated from a beef mince sample in Japan using Campylobacter coli , has a 12,587-bp genome encoding 18 putative coding sequences with an average GC content of 31.19%."

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"CAM-P21 was described to possess a broad host range, a better titer, and enhanced performance under diverse stress conditions compared to the Myoviridae family of phages."

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"Complete Genome Sequence of Campylobacter coli Bacteriophage CAM-P21."

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"CAM-P21 has a linear double-stranded DNA (dsDNA) genome 12,587 bp long with an average GC content of 31.19%."

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"Genomic DNA from CAM-P21 was extracted using the High Pure viral nucleic acid kit (Roche, Mannheim, Germany)."

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"The genomic DNA of phage CAM-P21 was completely sequenced with an Illumina HiSeq 4000 platform using the 2 × 150-bp paired-end strategy."

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"Genes associated with antibiotic resistance, toxins, and virulence factors were not found in the genome of CAM-P21."

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"Phage CAM-P21 was isolated from a beef mince sample collected in Fukuoka, Japan, using the double-layer agar method ( xref ) with C. coli CAN10 as the host strain (Fukuoka City Institute of Health and Environment), grown at 42°C in brain heart infusion (BHI) broth supplemented with 10 mM CaCl 2 under microaerobic conditions."