IndraLab

Statements

CALM1 binds PIP4K2A. 7 / 7
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sparser
"To better understand the interaction between CALM and PtdIns(4,5)P 2 -enriched membranes, we employed quartz crystal microbalance with dissipation monitoring (QCM-D), which is a highly sensitive analytical technique used to detect real-time changes in both mass and viscoelastic properties of solid-supported lipid bilayers."
40730637
sparser
"Our GIXD data show that CALM binding to PtdIns(4,5)P 2 -enriched monolayers induces tighter lipid packing, evidenced by a reduced area per lipid molecule (from 50 ± 1 to 63 ± 2 Å 2 )."
40730637
sparser
"Thus CALM must be able to bind simultaneously to PtdIns4,5P 2 and VAMP8."
22118466
sparser
"Given that CALM ANTH can bind PtdIns4,5P 2 and a small R-SNARE VAMP simultaneously and based on the structural identification of the binding sites for a VAMP and PtdIns4,5P 2 , the following deductions can be made."
22118466
sparser
"Langmuir tensiometry and NR were therefore used to analyse the interaction between the CALM mutant lacking the AH0 domain (CALM ΔAH0 ) and PtdIns(4,5)P 2 -enriched model monolayers."
40730637
sparser
"This allows the calculation of CALM:PtdIns(4,5)P 2 stoichiometry, as well as the protein volume inserted in the membrane per lipid molecule."
40730637
sparser
"Considering that PtdIns(4,5)P 2 is 10% in molecules of the total lipid composition, we estimate that each CALM wt molecule interacts with a cluster of 4-5 PtdIns(4,5)P 2 molecules."
40730637