IndraLab

Statements

OTUB1 binds TGFBI. 14 / 14
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sparser
"The OTUB1-TGFBI regulatory axis plays a crucial role in angiogenesis."
40430059
sparser
"These results establish that curcumol inhibits tumor angiogenesis by disrupting the OTUB1-TGFBI axis through enhanced ubiquitination and the degradation of TGFBI."
40430059
sparser
"Recent studies have identified a specific binding interaction between OTUB1 and TGFBI that is essential for OTUB1-mediated deubiquitination of TGFBI [ xref ]."
40430059
sparser
"However, the current research has several limitations: (1) insufficient mechanistic validation (e.g., lack of loss-of-function experiments via OTUB1 knockout); (2) undefined site specificity (unidentified critical ubiquitination sites mediating OTUB1-TGFBI interaction); and (3) unverified direct binding between curcumol and OTUB1 or its enzymatic activity modulation, which requires further validation through binding assays (e.g., surface plasmon resonance [SPR]) or enzyme activity tests."
40430059
sparser
"Immunofluorescence staining demonstrated strong OTUB1-TGFBI co-localization in control tumors ( xref G), with OTUB1 distributed in nuclear/cytoplasmic compartments and TGFBI localized to the extracellular matrix and membrane regions."
40430059
sparser
"Curcumol treatment significantly attenuated both protein expression and its co-localization (** p < 0.01; xref H), indicating that it disrupts OTUB1-TGFBI interactions that may be critical for tumor angiogenesis."
40430059
reach
"Half-life assays and ubiquitination test were performed to investigate the interaction between TGFBI and OTUB1."
36994729
sparser
"Importantly, the OTUB1-TGFBI interaction may represent a potential therapeutic target for mediating the anti-angiogenic effects of curcumol."
40430059
sparser
"Future studies should employ structural biology analyses of OTUB1-TGFBI protein interaction, biochemical validation of direct curcumol–OTUB1 binding, and dose-dependent evaluation of angiogenesis inhibition in both in vitro and in vivo models to refine the mechanistic understanding."
40430059
biogrid
No evidence text available
36994729
biogrid
No evidence text available
36994729
biogrid
No evidence text available
36994729
biogrid
No evidence text available
36994729
sparser
"Half-life assays and ubiquitination test were performed to investigate the interaction between TGFBI and OTUB1."
36994729