IndraLab

Statements

EPG5 binds USP8. 15 / 15
3 | 3 9
reach
"To examine whether USP8 binds to EPG5 in cells, we transfected Flag-tagged Epg5 and/or HA-tagged Usp8 in HEK293T cells and performed coimmunoprecipitation (co-IP) assays."
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"The results showed that USP8 is immunoprecipitated by EPG5, indicating that there is an interaction between EPG5 and USP8 (Fig. 3b)."
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"These data support that EPG5 binds to USP8."
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sparser
"In addition, USP8 binds to the coiled-coil domain of EPG5 and directly removes nonclassical K63-linked ubiquitin chains from EPG5 at lysine 252 to preserve autophagy flux in ESCs and maintain stemness ( xref )."
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"Co-IP assay confirmed the interaction between EPG5 and USP8 in ESCs (Fig.  xref )."
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"Furthermore, immunofluorescence microscopy showed that EPG5 did directly interact with USP8 in ESCs (Fig.  xref )."
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sparser
"These data support that EPG5 binds to USP8."
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"This work uncovers a novel crosstalk pathway between ubiquitination and autophagy through USP8-EPG5 interaction to regulate the stemness of ESCs."
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sparser
"EPG5 directly interacts with USP8."
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sparser
"To examine whether USP8 binds to EPG5 in cells, we transfected Flag-tagged Epg5 and/or HA-tagged Usp8 in HEK293T cells and performed coimmunoprecipitation (co-IP) assays."
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sparser
"The results showed that USP8 is immunoprecipitated by EPG5, indicating that there is an interaction between EPG5 and USP8 (Fig.  xref )."
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biogrid
No evidence text available
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biogrid
No evidence text available
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biogrid
No evidence text available
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sparser
"To further confirm that EPG5 interacts with USP8 in ESCs, stable ESC lines harboring HA- Epg5 or Myc- Usp8 vectors were established."
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