IndraLab

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COPS6 binds EGFP. 4 / 4
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"In our bleaching experiments, we used the localization of mCherry-tagged MKLP1 as a fiduciary for the position of the midbody and employed a high intensity 488 nm laser to simultaneously bleach mCherry-MKLP1 and EGFP-CSN6 (or EGFP) at the midbody."
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"By plotting the mean intensity of the fluorescent signal for EGFP and EGFP-CSN6, and curve fitted using a non-linear regression and the exponential one-phase association model, it was found that CSN6 has a larger immobile fraction than EGFP ( xref )); a result consistent with a strong association with substructures within the midbody such as the contractile ring."
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"Fluorescence recovery of EGFP was very rapid, while EGFP-CSN6 and mCherry-MKLP1 only recovered partially over the 5-min time period observed."
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"Therefore, to further characterize the association of the CSN with the midbody, we examined the localization and diffusion of EGFP-CSN6 as a marker of the COP9 signalosome in HeLa cells by fluorescence recovery after photobleaching (FRAP) ( xref )."
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