IndraLab

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"Immunoblot analysis revealed that ubiquitin chains were completely degraded by AMSH but neither by OTUB1 nor the GST control, further supporting our finding that TRIAD3 synthesises K63 linked ubiquitin chains in vitro, full length TRIAD3 protein as well as the TRIAD3-RBR 845 fragment."

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"Depletion of AMSH, which alters the balance of E3 ligase and DUB activity in the endosomal system, enhances the degradation of EGFR, presumably by inhibiting the removal of ubiquitin from EGFR and thereby preventing its ability to recycle."