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OTUD7B binds Ad. 16 / 16
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"After 24 h, Ad-Cezanne and Ad-shCezanne without GFP labelling were also transfected into cells, followed by 24 h treatment of DOX (1 μM)."
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"The stock solutions of Ad-OTUD7B and Ad-v were at concentrations of 1.58 × 10 10 and 4.00 × 10 10 plaque formation units (PFUs)/ml, respectively."
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"At the same time, adenovirus overexpressing Cezanne (Ad-Cezanne) was also constructed with Ad-v as a control."
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"Caspase-3 activity assay also confirmed that Ad-Cezanne could inhibit DOX-induced apoptosis while Ad-shCezanne exacerbated the phenomenon."
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"Adenovirus based on the pDC315-EGFP vector carrying OTUD7B (Ad-OTUD7B) and corresponding control (Ad-v) were synthesized by Hanbio Biotechnology, China."
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"Ad-OTUD7B and Ad-v were propagated in HEK293 cells."
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"Furthermore, we overexpressed OTUD7B by transfecting CFs with adenovirus, and the transfection efficiency is shown in Fig. 4 A. Treatment with Ad-OTUD7B showed decreased expression of α-SMA and collag[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Compared with the SCR group, CFs transfected with si-OTUD7B showed stronger migration ability, while in the Ad-OTUD7B group, the migration ability of CFs was weakened ( Fig. 5 A–B)."
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"To manipulate OTUD7B expression, we employed small interfering RNA (si-OTUD7B) to suppress OTUD7B expression and adenoviral vectors carrying OTUD7B-overexpressing plasmids (Ad-OTUD7B) to enhance its expression."
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"The CCK-8 assay revealed that si-OTUD7B treatment could promote the proliferation of fibroblasts, while Ad-OTUD7B could inhibit their proliferation ( Fig. 5 C)."
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"Moreover, Western blot showed that si-OTUD7B increased the expression of proliferating cell nuclear antigen (PCNA) and Cyclin D1 while Ad-OTUD7B decreased the expression of both of them ( Fig. 5 D)."
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"At the same time, we observed that Cezanne overexpression in NRCMs reduced the high level of ROS induced by DOX incubation while Cezanne knockdown increased it, as shown in the Fig. 3 D. Furthermore, [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The results revealed that si-OTUD7B could significantly increase the phosphorylation levels of FAK, ERK, and P38, while Ad-OTUD7B showed opposite effects ( Fig. 6 C–D)."
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"First, we investigated the changes in p-Smad2/3 levels after transfection with siRNA or Ad-OTUD7B, but no significant changes were found."
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"In contrast, DOX treatment induced the production of a large number of autophagosomes, which were mitigated by Ad-Cezanne transfection and exacerbated by Ad-shCezanne transfection ( Fig. 4 E)."
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"NRVMs infected with Ad-OTUD7B and Ad-HNF4α were treated with cycloheximide (CHX) to inhibit protein synthesis."
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