IndraLab

Statements

SNAI1 binds USP36. 22 / 22
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biogrid
No evidence text available
31911859
biogrid
No evidence text available
31911859
sparser
"Stable protein complexes between USP36 and Snail1 were readily detected in the nucleoli even in the presence of 500 mM NaCl (Fig.  xref )."
37833415
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"Lys157 of Snail1 is essential for Snail1-USP36 complex formation and ribotoxic stress-induced Snail1 nucleolar accumulation."
37833415
sparser
"To address whether USP36 binds to ubiquitinated Snail1, we employed a specific E1 inhibitor, TAK-243, to block protein ubiquitination."
37833415
sparser
"As shown in Supplementary Fig.  xref , USP36 effectively interacted with Snail1 regardless of the status of its ubiquitination."
37833415
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"To identify binding segments important for USP36-Snail1 interaction, we constructed a series of truncation mutants."
37833415
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"As shown in Fig.  xref , the N-terminus of USP36 (1–423 aa) interacted with the C-terminus of Snail1 (152–264 aa)."
37833415
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"Indeed, further experiments showed that the C2H2-type 1 Zinc finger (154–176 aa, ZnF1) of Snail1 protein is responsible for USP36-Snail1 interaction (Fig.  xref )."
37833415
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"By contrast, ectopic expression of ATXN3 upregulated both Snai11 WT and Snail1 K157R (Fig.  xref ), suggesting that K157 is critical for Snail1-USP36 interaction."
37833415
sparser
"Notably, while Snai11 WT and Snail1 K157R exhibited a similar half-life in the absence of ribotoxic stress, HHT significantly prolonged the protein half-life of Snai11 WT , but not Snail1 K157R (Fig.  xref ), indicating that USP36–Snail1 interaction is critically important in the stabilization of Snail1 in the nucleoli upon ribotoxic stress."
37833415
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"Next, we investigated whether USP36-Snail1 interaction is required for Snail1 nucleolar accumulation."
37833415
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"Together, these results indicate that K157 of Snail1 is essential for Snail1-USP36 protein complex formation and Snail1 nucleolar accumulation upon ribotoxic stress."
37833415
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"Activation of nucleolar USP36-Snail1 axis promotes ribosome biogenesis to promote cancer cell survival in response to ribotoxic stress."
37833415
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"We next investigated the biological significance of the nucleolar USP36-Snail1 axis."
37833415
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"Our abovementioned data indicate that activation of the nucleolar USP36-Snail1 axis promotes ribosome biogenesis to sustain cancer cell survival in response to ribotoxic stress, exemplified by HHT."
37833415
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"Additionally, USP36 interacts with snail family transcriptional repressor 1 (SNAIL1) to remove the polyubiquitin chains at positions K146 and K206 of the SNAIL1, achieving the stabilization of SNAIL1 and consequently upregulating the transcriptional synthesis of 47S pre-rRNA ( xref )."
40909126
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"USP36-Snail Nucleolar Axis."
40362410
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"We demonstrate that while Lys157 of Snail1 is critical for USP36-Snail1 protein complex formation, Lys146 and Lys206 on Snail1 are two key amino acid residues deubiquitinated by USP36."
37833415
reach
"Lys157 of Snail1 is essential for Snail1-USP36 complex formation and ribotoxic stress-induced Snail1 nucleolar accumulation."
37833415
reach
"Since the USP36 protein bears several NoLS , it is plausible that USP36 binds to and shuttles Snail1 into the nucleolus and stabilizes Snail1."
37833415
sparser
"The nucleolar USP36–Snail axis includes another crucial player component, ribosomal regulatory protein 1 (RRS1) [ xref ]."
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