IndraLab

"The two-pore (2P) domain K + channels (TREK-1 and TRAAK) are also activated by LPC and LPI, while only weakly responding to small-headed LPLs (LPE and LPS) [89] ."

"These results indicate that the effect of lysophospholipids on TREK channels is very dependent on the recording technique and warns of the extreme care that must be taken when interpreting negative results under some experimental conditions.Macroscopic currents: LPC, lysophosphatidylinositol (LPI), platelet-activating factor and Lyso-platelet-activating factor strongly activated TREK1 channels and induced a dose-dependent outward current in COS cells transfected with TREK1 channels and voltage-clamped at 0 mV."

"Consistent with this perspective, the LPC activated TREK1 channel is activated by general anaesthetics XREF_BIBR."

"In fact, LPA strongly and reversibly reduced TREK1 currents expressed in oocytes and inhibited the TREK1 currents induced by AA and LPC, with all these effects involving the activation of the Phospholipase C pathway and requiring the C-terminus of the channel protein (Cohen et al., 2009)."

"LPC activates TREK from the extracellular side while lysophosphatidic acid (LPA) activates from the intracellular layer."

"Polyunsaturated fatty acids (PUFAs) such as Arachidonic acid (AA), alpha-linolenic acid (ALA), docosahexaenoic acid (DHA) or lysophospholipids (LP) such as lysophosphatidylcholine (LPC) also activate TREK-1 channels and are neuroprotective against both ischemia and seizures induced by kainate injections [22], [29]."

"Additional TREK-1 activation in neurons by LIN or LPC would provide further protection [ xref ]."

"LPC activates TREK from the extracellular side while lysophosphatidic acid (LPA) activates from the intracellular layer. xref , xref LPC even becomes inhibitory in the inner leaflet. xref Phospholipids, such as phosphatidic acid (PA) and phosphatidylserine (PS), can also alter TREK channel activity. xref Phosphatidylinositol-4,5-bisphosphate (PI(4,5)P 2 ) can either activate or inhibit TREK1 depending on the intracellular pH and location in the bilayer. xref − xref Insertion of PIP 2 into the intracellular side of the membrane restores TREK1 activity. xref , xref The interaction between the C-terminus of TREK1 (and part of TM4) and PI(4,5)P 2 is thought to be nonspecific electrostatic binding. xref "

"As expected xref we observed activation of TREK1 by LPC or convex membrane curvature induced by negative pressure applied to cell-attached patches; however, although expression of TRPC5 conferred greater current in response to negative pressure, the current lacked the hallmark I – V of TRPC5 xref ."

"As expected XREF_BIBR we observed activation of TREK1 by LPC or convex membrane curvature induced by negative pressure applied to cell attached patches; however, although expression of TRPC5 conferred greater current in response to negative pressure, the current lacked the hallmark I-V of TRPC5 XREF_BIBR."