IndraLab

Statements

UBD binds OTUB1. 17 / 19
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reach
"This approach allowed us to directly measure K48 diubiquitin binding to the OTUB1 : E2 complex without dilution of the E2."
32049508
reach
"Moreover, our work indicates that this effect could be directly mediated by the binding of phospho-FAT10 to OTUB1, which activates this DUB leading to enhanced deubiquitylation of TRAF3."
37940187
reach
"Furthermore, the non covalent interaction between FAT10 and OTUB1 not only enhances its isopeptidase activity towards K48 linked ubiquitin moieties but also strengthens its non catalytic activity in reducing K63 polyubiquitylation of its target protein TRAF3."
30718280
sparser
"In the absence of E2, we found that OTUB1 binds K48 diubiquitin with a K d of 84 μM ( xref ), which is within the range of K M values reported here ( xref ) and previously xref , xref ."
32049508
sparser
"We determined equilibrium dissociation constants for OTUB1 (C91S) binding to K48 diubiquitin in the presence of UBE2D1, UBE2D3, and UBE2N ( xref – xref ; thermodynamic parameters listed in xref )."
32049508
sparser
"Therefore, we performed a FLAG immunoprecipitation combined with an immunoblot analysis to study the interaction of OTUB1 and WT FAT10 or the FAT10 phospho-mutants in HEK293 cells ( xref )."
37940187
sparser
"This result, which is in line with the experiment shown in xref , suggests that FAT10 phosphorylation is important for the OTUB1–FAT10 interaction."
37940187
biogrid
No evidence text available
30718280
sparser
"Unexpectedly, the prominent non-covalent binding of FAT10 to OTUB1 leads to both the activation of the deubiquitylating activity of OTUB1 and the non-enzymatic inhibition of E2 enzymes."
32586037
sparser
"To further confirm that the negative effect that FAT10 has on the IFN-I response is mediated by binding of phosphorylated FAT10 to OTUB1, we measured the secretion of IFN-β in A549 wt and A549 OTUB1-deficient cells in the presence of WT FAT10 or phospho-mimetic FAT10 (FAT10 E)."
37940187
sparser
"In the absence of a structure of OTUB1 bound to Lys48 diubiquitin and E2, we took advantage of the observation that the two ubiquitins bound to the proximal and distal sites of OTUB1 mimic the binding of Lys48 diubiquitin xref , xref to examine the structural effect of E2 binding on the N-terminal ubiquitin binding helix of OTUB1."
23955022
sparser
"This structure was therefore used as a model for OTUB1 bound to a true Lys48-linked diubiquitin substrate and UBCH5B."
23955022
sparser
"In particular, it was shown that FAT10 binding to either wild-type OTUB1 or the enzymatically inactive C91S variant of OTUB1 downregulated the poly-ubiquitylation of TRAF3 in cells, and this effect was especially prominent for ubiquitin chains linked via the lysine residue 63 of ubiquitin (K63) [ xref ]."
32586037
sparser
"Moreover, our work indicates that this effect could be directly mediated by the binding of phospho-FAT10 to OTUB1, which activates this DUB leading to enhanced deubiquitylation of TRAF3."
37940187
sparser
"FAT10 phosphorylation increases the binding of FAT10 to the TRAF3-deubiquitylase OTUB1 and its FAT10-mediated activation."
37940187
sparser
"Thereby, FAT10 phosphorylation strengthens the FAT10–OTUB1 interaction, causing a reduced polyubiquitylation of TRAF3 which strongly correlates with the reduced antiviral IFN-I secretion caused by phospho-FAT10."
37940187
sparser
"Moreover, the interaction of FAT10 with OTUB1 stabilizes the deubiquitylating enzyme, leading to the down-regulation of type-I interferon production, a key component of the antiviral response ( xref )."
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