IndraLab

"In light of these findings, it could be suggested that YAP1 silencing could reverse the protective effects of SPC on H/R-treated H9c2 cells.Altogether, our findings indicated that SPC up-regulated USP22, which stabilized KDM3A protein level via deubiquitination, and KDM3A inhibited H3K9me2 levels in the YAP1 promoter region to encourage YAP1 transcription, leading to reduction of MI/RI."

"However, we failed to elucidate whether SPC could directly up-regulate USP22 to target KDM3A."

"USP 22, KDM3A, and YAP1 were found to be down-regulated in MI/RI and SPC protected MI/RI rats, as evidenced by up-regulated expressions of USP22, KDM3A, and YAP1, reduced pathological injury in the myocardium, myocardial infarction areas, apoptosis, and levels of CK-MB, cTnI, and LDH, and enhanced H9c2 cell viability; while the protective effects of Sevo were counteracted by silencing of USP22, KDM3A, and SPC upregulated USP22, which stabilized KDM3A protein levels via deubiquitination, and KDM3A inhibited histone 3 lysine 9 di-methylation (H3K9me2) levels in the YAP1 promoter to encourage YAP1 transcription, to reduce MI/RI."