IndraLab

Statements

USP8 binds Flag. 19 / 19
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"The results of Co-IP and western blot revealed that there was an exogenous specifical interaction between Flag-USP8 and His-PD-L1 (Fig. xref )."
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"A FANCD2 interaction with the highly expressed Flag-USP8 or with the lower-expressed VDUI could not be detected, indicating that the observed USP1/FANCD2 interaction is specific ( Figure 4C , middle a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15694335
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"Exogenous in vivo ubiquitination assay revealed that the polyubiquitination level of His-PD-L1 was obviously decreased in Flag-USP8 transduced HEK-293T cells (Fig. xref )."
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"Besides, we mutated K6, K11, K27, K29, K33, K48, K63 lysine residues on the ubiquitin to arginine (K6R, K11R, K27R, K29R, K33R, K48R, K63R) and co-transfected His-PD-L1, Flag-USP8, and HA-Ub (including wide type and mutated type) into HEK-293T cells."
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"To validate this notion, we stably transfected Flag-USP8 into UROtsa( SNHG1 ), while shRNAs specifically targeting USP8 (shUSP8#1 and shUSP8#2) were stably introduced into U5637(sh SNHG1 #1)."
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"Our data demonstrated that ectopic expression of Flag-USP8 restored PTEN levels in UROtsa( SNHG1 ) cells, while USP8 knockdown attenuated PTEN levels in U5637(sh SNHG1 #1) cells (Fig.  xref C and D)."
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"As shown in Fig.  xref I, PTEN was presented in the immune-complex pulled down of Flag-USP8 using anti-USP8 antibodies, strongly indicating the interaction USP8 enzyme with PTEN protein."
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"Flag-USP8 interacted with HA-TAB2 ( xref G, lane 4) or Myc-TAK1 ( xref H, lane 4)."
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"The ubiquitination of TAB2 and TAK1 was markedly attenuated in the presence of Myc-USP8 or Flag-USP8 in a dose-dependent manner ( xref I, lane 4, 6 ; xref J, lane 4, 6 ), as compared with that in the absence of Myc-USP8 or Flag-USP8 ( xref I, lane 3; xref J, lane 3)."
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"Then, to verify the results of proteomics and ubiquitinome, we co-transfected Flag-USP8 and Myc-NBR1 into HEK-293T cells."
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"Flag-USP8 interacted with Myc-BECN1 ( xref A, lane 4)."
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"Moreover, Myc-BECN1 was ubiquitinated in the absence of Flag-USP8, whereas significant deubiquitination of Myc-BECN1 could observed in the presence of Flag-USP8 in a dose-dependent manner ( xref B, lane 4–6 vs. lane 3), indicating that USP8 induces the de-ubiquitination of BECN1."
34688043
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"The results of Co-IP and western blot revealed that there was an exogenous specifical interaction between Flag-USP8 and Myc-NBR1 (Fig. xref )."
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"In addition, Flag-USP8 interacted with Myc-p62 ( xref D, lane 4)."
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"Myc-p62 was ubiquitinated in the absence of Flag-USP8 ( xref E, lane 3), whereas marked deubiquitination could be seen in the presence of Flag-USP8 in a dose-dependent manner ( xref E, lane 4–6 vs. lane 3), indicating that USP8 induces the deubiquitination of p62."
34688043
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"Exogenous in vivo ubiquitination assay revealed that the polyubiquitination level of Myc-NBR1 was markedly decreased in Flag-USP8 transduced HEK-293T cells (Fig. xref )."
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"To identify which lysine residues of ubiquitin are involved in USP8 de-ubiquitination process of NBR1, we mutated K6, K11, K27, K29, K33, K48, K63 lysine residues on the ubiquitin to arginine (K6R, K11R, K27R, K29R, K33R, K48R, K63R) and co-transfected Myc-NBR1, Flag-USP8, and HA-Ub (including wide type and mutated type) into HEK-293T cells."
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"To identify whether K767 (Lys-767) and K887 (Lys-887) lysine sites on the NBR1 identified by ubiquitinome are involved in USP8-mediated de-ubiquitination process, we mutated K767 and K887 lysine to arginine on the NBR1 and co-transfected Myc-NBR1 (including wide type and K767R-mutation and K887R-mutation), Flag-USP8, and HA-Ub into HEK-293T cells."
40410130
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"To address this problem, we co-transfected Flag-USP8 and His-PD-L1 into HEK-293T cells."
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