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USP29 decreases the amount of TWIST1. 6 / 6
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"As shown in Figure 2F, USP29 WT but not its catalytically inactive mutant C294S could dramatically decrease the ubiquitination level of TWIST1."
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"Consistently, the depletion of USP29 in MDA‐MB‐231 and BT549 cells markedly increased the ubiquitination level of TWIST1 (Figure 2H, Figure S2I, Supporting Information)."
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"In addition, the overexpression of USP29 dramatically decreased the ubiquitination level of TWIST1, which was markedly blocked by the treatment of RO‐3306 or the depletion of CDK1 (Figure 6B,C)."
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"As shown in Figure 6D and Figure S6B (Supporting Information), the overexpression of USP29 WT could dramatically decrease the ubiquitination level of TWIST1, whereas the 3A mutant failed to do so."
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"We found that the reduced TWIST1 protein level in USP29‐deficient cells was rescued by the reconstitution of USP29 WT, but not the 3A mutant (Figure S6C,D, Supporting Information)."
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"Both USP29 and TWIST1 are overexpressed in TNBC tumor specimens, but not in normal adult breast tissues, highlighting the potential of USP29 as a more druggable target toward TWIST1 stability in TNBC.It is also noteworthy that we can not rule out the possibility of additional mechanisms engaged in UPS29‐regulated tumor progression of TNBC since the ectopic expression of TWIST1 could not completely rescue functional alterations caused by the depletion of USP29 in TNBC cells."
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