IndraLab

Statements

OTUD1 binds Flag. 16 / 16
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"HEK-293T cells were co-transfected with plasmids encoding Flag-OTUD1, GFP-PRDX1, and HA-tagged ubiquitin, followed by treatment with proteasome inhibitor MG132."
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"The exogenous interaction between OTUD1 and AMPKα2 was verified in Hek293T cells expressing Flag-OTUD1 and HA-AMPKα2 (Fig.  xref )."
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"Furthermore, His-RIP2 and Flag-OTUD1 were transfected into HEK293T cells, Co-IP experiment and confocal microscopy observed the interaction and colocalization of His-RIP2 with Flag-OTUD1 (Fig.  xref C, D)."
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"p53 was specifically immunoprecipitated with OTUD1 by the Flag M2 bead in U2OS cells overexpressed with Flag-OTUD1 and Myc-p53 ( Fig. 3 A )."
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"Further, p53 was also specifically immunoprecipitated with OTUD1 by the p53 antibody, but not control IgG, in U2OS cells overexpressed with Flag-OTUD1 ( Fig. 3 B)."
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"GST‐tagged AIF was confirmed to be directly associated with Flag‐OTUD1, and vice versa (Figure  xref )."
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"In parallel, after transfecting Flag-OTUD1 plasmids into Hek 293T cells, β-catenin was successfully precipitated using an anti-Flag antibody, whereas no precipitation was observed with the control IgG antibody ( xref C)."
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"We transfected NVRMs with Flag-OTUD1 to express OTUD1 and then challenged the cells with Ang II in the presence or absence of STAT3 inhibitor."
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"MS was performed as previously described. [ xref ] Briefly, Flag‐OTUD1 was transfected into 293T cells, and cell lysates were immunoprecipitated with anti‐Flag magnetic beads."
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"We transfected Flag-OTUD1 and Myc-HK2 plasmids into HEK-293T cells, and showed that OTUD1 interacts with HK2 following Co-IP assay (Fig.  xref E-F)."
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"U2OS, HCT116, or 293T cells were transfected with vectors expressing Flag-OTUD1, HA-Ub, or Myc-p53, as indicated."
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"Neither overexpression of OTUD1 by transfected Flag-OTUD1 nor knockdown of OTUD1 by siRNA in HEK293T cells showed any significant change in protein level of RIP2, indicating OTUD1 did not affect RIP2 protein degradation (Fig.  xref C, D)."
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"OTUD1 was silenced using siRNA (siOTUD1) or overexpressed by transfection of the Flag-OTUD1 plasmid (OTUD1 OE ) in NRPCs (Supplementary Fig.  xref )."
40683882
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"Although Flag-OTUD1 C320S bound to PRDX1 (Figure xref C), it did not deubiquitinate PRDX1 in HEK-293T cells (Figure xref D), indicating that OTUD1 reversed the ubiquitination of PRDX1 through the C320 active site."
40585986
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"No notable changes were observed in the degradation rate of CARD9 protein in HEK‐293T transfected with Flag‐OTUD1 (Figure  xref )."
39118286
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"Overexpressing Flag‐OTUD1 in HEK 293T cells did not increase the protein level of CARD9 (Figure  xref )."
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