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USP35 increases the amount of SLC7A11. 5 / 5
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"USP35 knockdown reduced the protein level of cystine/glutamate transporter SLC7A11 (Fig. 3A)."
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"Conversely, overexpression of USP35 also increased the SLC7A11 protein level (Fig. 3B), indicating that USP35 is important for SLC7A11 expression in ER+ breast cancer cells."
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"However, MG132 (a proteasome inhibitor) treatment could not rescue the SLC7A11 protein level that was inhibited by USP35 knockdown (Supplementary Fig. 4), indicating that USP35 did not upregulate SLC7A11 through deubiquitination."
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"Conversely, although USP35 overexpression increased SLC7A11 protein level compared with vector control, BRD4 inhibitor (+)-JQ-1 treatment reduced the increase in SLC7A11 protein level induced by USP35 overexpression (Fig. 6D)."
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"Furthermore, immunohistochemistry analysis revealed that USP35 increased the levels of SLC7A11, whereas (+)-JQ-1 treatment significantly reduced the levels of SLC7A11, and increased the level of 4-HNE, a marker of ferroptosis, in ER+ breast tumors (Fig. 7F, G)."
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