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"The binding affinity between PFKFB3 and OTUD4 was assayed by a Biacore T200 (GE Healthcare)."

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"Co-IP assay demonstrated that TGF-β1 treatment promoted the interaction of endogenous PFKFB3 and OTUD4 (Fig. 6d, e)."

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"Consistently, immunofluorescence examination (Fig. 6f) and PLA assay (Fig. 6g) showed the enhanced interaction between OTUD4 and PFKFB3 in TGF-β1 treated NRCFs."

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"Herein, we showed that TGF-β1 promoted the interaction of OTUD4 and PFKFB3."

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