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USP48 binds HMGA2. 17 / 17
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"Although we failed to identify a conserved SIM motif in HMGA2, our findings demonstrated that the SUMOylated USP48 could still strongly interact with HMGA2, thereby enhancing the affinity of USP48 for ubiquitin chains and augmenting its DUB activity."
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"The signaling pathway mediated by the USP48–HMGA2 axis plays a critical role in promoting invasion and metastasis of CRC cells."
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"To further assess the clinical significance of the USP48-HMGA2 axis and establish their correlation in CRC, we performed IHC staining to examine the expression of these proteins in consecutive sections of tissue microarrays (TMAs) consisting of 90 CRC tissues and 86 adjacent normal tissues."
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"Furthermore, the interaction between endogenous USP48 and HMGA2 was validated through an in-situ proximity ligation assay (PLA) in DLD1, SW480, and HCT116 cells ( xref G and xref )."
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"Taken together, these results demonstrate the efficacy of DUB-IN-2 in inhibiting USP48 enzyme activity and antagonizing USP48-HMGA2 signaling during CRC metastasis."
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"These findings suggest a specific interaction between USP48 and HMGA2, indicating that USP48 directly binds to HMGA2."
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"We have identified an interaction between USP48 and HMGA2, and our study further investigated the involvement of USP48 in maintaining the stability of HMGA2."
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"USP48 interacts with HMGA2."
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"Co-immunoprecipitation (Co-IP) assays were used to investigate the interaction between USP48 and HMGA2 in vivo ."
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"To investigate the impact of SUMOylation on USP48's enzymatic activity as a deubiquitinating enzyme for HMGA2, we assessed the interaction between HMGA2 and USP48."
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"These findings suggest a specific interaction between USP48 and HMGA2, indicating that USP48 directly binds to HMGA2.3.2 USP48 maintains HMGA2 stability."
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"We have identified an interaction between USP48 and HMGA2, and our study further investigated the involvement of USP48 in maintaining the stability of HMGA2."
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"Co-immunoprecipitation (Co-IP) assays were used to investigate the interaction between USP48 and HMGA2 in vivo."
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"Moreover, purified GST-HMGA2 exhibited the ability to directly interact with HA-tagged USP48 under cell-free conditions, as opposed to GST control (Fig. 1E), indicating a direct binding between HMGA2 and USP48."
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"Furthermore, the interaction between endogenous USP48 and HMGA2 was validated through an in-situ proximity ligation assay (PLA) in DLD1, SW480, and HCT116 cells (Fig. 1G and Fig. S1D)."
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"To investigate the impact of SUMOylation on USP48's enzymatic activity as a deubiquitinating enzyme for HMGA2, we assessed the interaction between HMGA2 and USP48."
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"Our findings indicate that compared to USP48-WT, the interaction between HMGA2 and USP48-K258R mutant was relatively weak ( xref C)."
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