IndraLab

Statements

OTUD1 binds PRDX4. 11 / 11
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"Thus, the OTUD1–PRDX4 axis seems to be a crucial target for proteasome-inhibitor-independent therapy for MM."
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"However, a precise mechanism how OTUD1-PRDX4 axis mediates the Ig synthesis remained unclear."
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"In summary, our work unveils the OTUD1-PRDX4 axis as a yet undescribed regulatory pathway driving Ig production and PI sensitivity in MM cells and highlights importance of OTUD1 in myeloma proliferation (Fig.  xref )."
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"At the same time, OTUD1 binds and elevates PRDX4 protein amount."
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"Co-immunoprecipitation of OTUD1 and PRDX4 complexes was performed by lysing RPMI8226 cells in immunoprecipitation buffer (20 mM TRIS, pH 7.5, 150 mM NaCl, 0.5% NP40) containing phosphatase and protease inhibitor cocktail (Thermo Fisher Scientific), 20 mM N-ethylmaleimide, and 5 mM iodacetamide for 1 h on ice."
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"Initially, we validated the OTUD1-PRDX4 interaction in series of reciprocal co-immunoprecipitations with both tagged and endogenous proteins (Fig.  xref )."
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"To map and localize the OTUD1-PRDX4 interaction, we used the full length (FL) and the Δ1-38 PRDX4 constructs."
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