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BRCC3 binds ZEB1. 4 / 4
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"Since we confirmed that ZEB1 expression is regulated by BRCC3, we further verified the interaction between BRCC3 and ZEB1 using a co-IP assay."
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"After MDA-MB-231 cells were transfected with Myc-BRCC3 and treated with MG132, ZEB1 was pulled down by co-IP with an anti-Myc antibody ( Figure 3B), which indicated the interaction between BRCC3 and ZEB1."
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"In the present study, we identify BRCC3, a DUB that binds to ZEB1, but the role of BRCC3 in TNBC proliferation and metastasis has not yet been reported."
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"To examine the interaction between endogenous BRCC3 and ZEB1 in MDA-MB-231 cells, 20 μL of Protein A/G Agarose was mixed with 5 μL of anti-ZEB1 antibody (ab276129; Abcam) or anti-BRCC3 antibody (ab115172; Abcam) and incubated for 2 h at room temperature, after which the supernatant was added and incubated overnight at 4°C. IgG was used as a negative control."
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