IndraLab

Statements

OTUB1 binds GST. 5 / 5
| 1 4
sparser
"The K d for binding of UBE2N to OTUB1 was 8.1 μM, which is consistent with previous studies that measured a K d of 7.04 μM using fluorescence polarization xref and K d of 8.9 μM measured by surface plasmon resonance using a GST-OTUB1 fusion xref ."
32049508
sparser
"Glutathione-Sepharose beads were added to the lysates from E . coli BL21(DE3)(pGEX-4T1) and E . coli BL21(DE3)(pGST-OTUB1) to allow the binding of GST and GST-OTUB1 to the beads, respectively ( xref , lanes 1, 2)."
38814988
sparser
"In the in vitro ubiquitination assay, individual Flag-tagged substrates (MSH2, FOXM1, and DEPTOR) were incubated with GST-OTUB1 (WT, C91A, or D88A) and HA-Ub in a ubiquitination system containing rabbit reticulocyte lysate (RRL), as described previously ( xref ), and ubiquitinated substrates were visualized by western blotting using a Ub antibody."
33640455
sparser
"We found that the His-NS2-Ni 2+ -NTA agarose beads pulled down GST-OTUB1 ( xref , lane 4); however, GST-OTUB1 was not pulled down by His-Ni 2+ -NTA agarose beads, ( xref , lane 3)."
38814988
reach
"Glutathione-Sepharose beads were added to the lysates from E. coli BL21(DE3)(pGEX-4T1) and E. coli BL21(DE3)(pGST-OTUB1) to allow the binding of GST and GST-OTUB1 to the beads, respectively (Fig 4A, lanes 1, 2)."
38814988