IndraLab

Statements

CALM1 binds VGA1. 23 / 23
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"Since the pixels extracted by PSPNet lack rich contextual information, SA-FFNet xref introduces VH-CAM and UC-PPM to enhance the contextual information of pixels."
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"In mutant VH-CAM, Val37, Gly44, Leu45 and Trp47 in framework 2 were substituted by Phe37, Glu44, Arg45 and Gly47, respectively."
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"Purification yields of soluble protein from one liter of bacterial culture normalized to an A 550 of 10 were 0.9(±0.1) mg in the VH-W construct, 5(±0.1) mg in VH-CAM, and 8(±0.1) mg in VH-D. All modif[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To determine the in vitro relative affinity of the isolated VH domains for Vif protein, the amounts of anti-Vif VH, VH-W, VH-CAM and VH-D domains were normalized and analyzed by ELISA."
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"To determine the expression levels of single-domains in the reducing environment of the cytoplasm in mammalian cells, VH, VH-W, VH-CAM and VH-D were expressed in 293T cells and cell lyzates were prepa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The data in Figure 4 demonstrate that anti-Vif VH-CAM and VH-D antibody fragments were the most abundant intracellularly among the four single-domains."
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"Figure 5 shows that anti-Vif VH-CAM and anti-Vif VH-D were the most stable, showing the higher steady-state accumulation and increased half-life, compared to VH and VH-W. Moreover, it should be noted [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In contrast, the overall steady-state levels of anti-Vif VH and VH-W were much less than VH-CAM and VH-D domains with a relatively short protein half-life of about two hours."
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"These results were consistent with our observations using immunofluorescence microscopy where VH and VH-W consistently exhibited weaker fluorescence intensity than VH-CAM and VH-D, suggesting a lower [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Therefore, protein accumulation at steady-state correlates significantly with differences in intracellular protein stability of VH-CAM and VH-D. To provide a qualitative and quantitative measure of th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"47 Non-permissive H9 38 LTR-CAT cells were cotransfected with pSVCATΔ env ΔVif, pVSVG, pSVL-Vif and plasmids encoding anti-Vif scFv 4BL, VH, VH-W, VH-CAM or VH-D domains."
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"Co-expression of anti-Vif VH-CAM and VH-D domains in H9 38 cells reduced trans -complementation to 30% and 10% of the wild-type level, respectively."
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"These recombinant HIV-1 viruses were derived from pHIVnPLAP-IRES-N+, by replacing the human placental alkaline phosphatase (PLAP) gene by scFv 4BL, VH, VH-W, VH-CAM and VH-D, generating pHIV-4BL, pHIV[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Two patterns emerged in comparison with Vif-positive and Vif-negative virus in the presence of Apobec3G. First, the VH and VH-W constructs showed no obvious differences compared to HIV-1 plus Apobec3G[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"50,51 As shown in Figure 9C , a reduced effect in HIV infectivity was obtained with VH and VH-W. In contrast, VH-CAM, VH-D and scFv 4BL showed a pronounced reduction of 20–15% in viral infectivity com[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"As demonstrated, intrabodies VH-CAM and VH-D are far more effective and exhibited increased steady-state expression levels compared to VH and VH-W. One possible explanation for low steady-state accumu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"These results suggest that turnover rate, an index of overall protein stability, and steady-state accumulation of VH-CAM and VH-D are critical factors for the effectiveness of intrabodies."
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"16 For camelization, the anti-Vif VH domain was mutated into VH-CAM, VH-W and VH-D single-domains by protein engineering."
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"43 For the VH-CAM mutant, specific oligonucleotide primers encoding point mutations V37F, G44E, L45R and W47G, were used for PCR-based mutagenesis."
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"For the VH-D mutant, the same cloning procedure for VH-W construction was applied but starting from the VH-CAM mutant."
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"To evaluate expression of Apobec3G in the presence of Vif and antibody constructs, 293T cell lines were co-transfected where indicated with scFv 4BL, VH, VH-W, VH-CAM and VH-D, together with pcDNA3.1-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"47 Briefly, H9 38 LTR-CAT cells (10 6 ) and Jurkat (10 6 ) cells were cotransfected by Fugene (Roche), with 2 μg of pSVCATΔ env ΔVif, 2 μg of pVSVG, 2 μg of pSVLVif and either plasmids encoding anti-V[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To further evaluate the inhibition of HIV-1 infectivity by VH domains and scFv, 293T cells (1×10 6 –2×10 6 ) were cotransfected by Fugene (Roche), with 2 μg of pVSVG, 2 μg of HIV-1 NL4-3 , 2 μg of pcD[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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