IndraLab

Statements

HK2 binds OTUD1. 10 / 10
| 10
sparser
"Our findings address a key knowledge gap by elucidating the OTUD1-HK2 axis as a novel regulatory pathway in SAE, offering potential therapeutic targets to mitigate cognitive deficits in sepsis."
40500776
sparser
"The results of fluorescent colocalization and co-immunoprecipitation (Co-IP) subsequently verified the interaction between OTUD1 and HK2."
40500776
sparser
"Molecular docking, 3D confocal microscopy, and co-immunoprecipitation were conducted to detect the interaction between OTUD1 and HK2."
40500776
sparser
"Mechanistically, OTUD1 directly binds to the C-terminal domain of HK2 through its Ala-rich domain and selectively cleaves K63-linked polyubiquitin chains on HK2 to promote the dissociation of HK2 from mitochondria, thereby activating the NLRP3 inflammasome and pyroptosis."
40500776
sparser
"OTUD1 interacts with HK2."
40500776
sparser
"Such efforts will deepen our understanding of OTUD1-HK2 regulation in SAE."
40500776
sparser
"We transfected Flag-OTUD1 and Myc-HK2 plasmids into HEK-293T cells, and showed that OTUD1 interacts with HK2 following Co-IP assay (Fig.  xref E-F)."
40500776
sparser
"Future studies will focus on validating safety using conditional knockout models and developing selective inhibitors based on the interaction between OTUD1 and HK2."
40500776
sparser
"In addition, we validated this in BV2 cells, where HK2 was immunoprecipitated, and OTUD1 was detected in the immunoprecipitate by immunoblotting, confirming the endogenous interaction between OTUD1 and HK2 (Fig.  xref G)."
40500776
sparser
"To characterize the mechanism by which OTUD1 regulates the mitochondrial translocation of HK2, we used the HDOCK docking tool to predict the interaction between OTUD1 and HK2 firstly."
40500776