IndraLab

Statements


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"We observed a reduced ubiquitin signal with GST-UCHL3 but not with GST, suggesting that UCHL3 directly removed ubiquitin from Ku80."

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"Purified His-RAD51 was able to interact with GST-UCHL3 under cell-free conditions even with a relatively high salt concentration in the washing buffer ( xref C), suggesting a direct interaction between UCHL3 and RAD51."

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"The cDNA of UCH-L3 was subcloned from the library plasmid with GenBank N27190 by PCR into the Eco RI/ Xho I sites of pGEX-4T1 (Amersham) encoding for a GST-UCH-L3 fusion protein."

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"1 Experimental Models: Cell Lines HEK293T Laboratory of Michiel Vermeulen N/A HeLa Cells Spruijt et al., 2013 N/A IB10 Cells Spruijt et al., 2013 N/A NPCs Spruijt et al., 2013 N/A HEK293T-EGFP-TAB-NZF[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"