IndraLab

Statements

PLK1 phosphorylates USP16. 19 / 22
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rlimsp
"Plk1 phosphorylates and activates Usp16."
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sparser
"In vitro phosphorylation of Usp16 with single (S330A, S386A, or S486A) or collective 3A (S330A/S386A/S486A) mutation showed that Plk1 phosphorylated Usp16 at all three sites (Fig. S2 D)."
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sparser
"Collectively, our results strongly suggest that CDK1 promotes the interaction between Plk1 and Usp16 and enhances the phosphorylation of Usp16 by Plk1."
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sparser
"CDK1 and Plk1 sequentially phosphorylate and activate Usp16, which in turn deubiquitinates Plk1 to maintain the kinase’s kinetochore localization and promote proper chromosome alignment in mitosis."
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reach
"Collectively, our results strongly suggest that CDK1 promotes the interaction between Plk1 and Usp16 and enhances the phosphorylation of Usp16 by Plk1."
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sparser
"We found that the level of ubiquitinated histone H2A (ubH2A) was high in interphase and low in mitotic HeLa cells (Fig. S3, C–E), suggesting that Plk1 phosphorylates and activates Usp16."
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reach
"We found that the level of ubiquitinated histone H2A (ubH2A) was high in interphase and low in mitotic HeLa cells, suggesting that Plk1 phosphorylates and activates Usp16."
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rlimsp
"Collectively, our results strongly suggest that CDK1 promotes the interaction between Plk1 and Usp16 and enhances the phosphorylation of Usp16 by Plk1."
26323689
reach
"These results strongly suggest that Plk1 phosphorylates and activates Usp16, but the exact molecular mechanism of the activation is not clear at the moment."
26323689
sparser
"Plk1 phosphorylates and activates Usp16."
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rlimsp
"Then, Plk1 further phosphorylates Usp16 to activate it."
26323689
rlimsp
"These results strongly suggest that Plk1 phosphorylates and activates Usp16, but the exact molecular mechanism of the activation is not clear at the moment."
26323689
reach
"In vitro phosphorylation of Usp16 with single (S330A, S386A, or S486A) or collective 3A (S330A/S386A/S486A) mutation showed that Plk1 phosphorylated Usp16 at all three sites."
26323689
sparser
"Interestingly, when Plk1 inhibitor BI2536 was applied after the cells entered mitosis, the upshift of Usp16 was abolished ( xref ), suggesting that Usp16 was also phosphorylated by Plk1 in vivo."
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reach
"Interestingly, when Plk1 inhibitor BI2536 was applied after the cells entered mitosis, the upshift of Usp16 was abolished (XREF_FIG), suggesting that Usp16 was also phosphorylated by Plk1 in vivo."
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rlimsp
"Interestingly, when Plk1 inhibitor BI2536 was applied after the cells entered mitosis, the upshift of Usp16 was abolished (Fig. 3 G), suggesting that Usp16 was also phosphorylated by Plk1 in vivo."
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sparser
"Next, we set out to investigate the function of Usp16 phosphorylation by Plk1."
26323689
sparser
"These results strongly suggest that Plk1 phosphorylates and activates Usp16, but the exact molecular mechanism of the activation is not clear at the moment."
26323689
reach
"Plk1 phosphorylates and activates Usp16."
26323689