IndraLab

Statements

USP37 binds CDC73. 28 / 28
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"We have shown above that CDC73 is polyubiquitinated via K48-linked ubiquitin chains that are deubiquitinated by USP37, and that CDC73 directly binds to USP37, via an interaction between the β-catenin binding region of CDC73 and the ubiquitin-interacting motifs (UIM2 and 3) of USP37."
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"Purified USP37 interacted with CDC73, and vice versa ( xref B,C)."
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No evidence text available
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"They found that ubiquitin-specific proteases 37 (USP37) directly binds to parafibromin and prevents ubiquitin-induced degradation of parafibromin."
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"Repeated GST pull-down analysis using GST-USP37/pGEX-4T-1 and GST-CDC73/pGEX-4T-1 confirmed a direct interaction between USP37 and CDC73."
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No evidence text available
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"The biochemical interaction between the USP37 and CDC73 and their reciprocal binding domains were studied."
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"In co-IP assays using the FLAG antibody, the CDC73 mutants did not interact with USP37, whereas wild-type CDC73 did ( xref B)."
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"Therefore, it is presumed that CDC73 binds directly to USP37."
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"As a result, it was confirmed that full-length CDC73 binds to full-length USP37 and Δ3 and Δ4 that contain UIM2 and 3 ( xref B)."
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"CDC73 Binds Directly to USP37."
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"To confirm the binding between USP37 and CDC73 shown in the yeast two-hybrid screen, a GST pull-down and co-IP analysis was performed."
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"This revealed that purified GST-USP37 bound to FLAG-CDC73 (Figure 2B) and purified GST-CDC73 bound to Myc-USP37 (Figure 2C) in E. coli strain BL21."
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"Reciprocal binding between CDC73 and USP37 was also detected in co-IP experiments performed in COS7 cells using FLAG-CDC73 and Myc-USP37 (Figure 3B)."
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"We conclude that CDC73 binds directly to USP37."
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"We investigated the domain of CDC73 that binds USP37, and vice versa."
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"Full-length CDC73 bound to full-length USP37 and its Δ3 and Δ4 derivatives containing ubiquitin-interacting motifs (UIMs) 2 and 3 (Figure 5B)."
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"Similarly, full-length USP37 bound to full-length CDC73 and its Δ1, Δ2 and Δ5 derivatives containing the β-catenin binding region (Figure 5C)."
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"The biochemical interaction between the USP37 and CDC73 and their reciprocal binding domains were studied."
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"We have shown above that CDC73 is polyubiquitinated via K48-linked ubiquitin chains that are deubiquitinated by USP37, and that CDC73 directly binds to USP37, via an interaction between the β-catenin binding region of CDC73 and the ubiquitin-interacting motifs (UIM2 and 3) of USP37."
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"Repeated GST pull-down analysis using GST-USP37/pGEX-4T-1 and GST-CDC73/pGEX-4T-1 confirmed a direct interaction between USP37 and CDC73."
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"Therefore, it is presumed that CDC73 binds directly to USP37."
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"As a result, it was confirmed that full-length CDC73 binds to full-length USP37 and Δ3 and Δ4 that contain UIM2 and 3 (Figure 5B)."
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"CDC73 Binds Directly to USP37."
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"We investigated the domain of CDC73 that binds USP37, and vice versa."
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