IndraLab

Statements

OTUB1 binds UBE2D1. 24 / 26
8 | 9 7
sparser
"Consistently, Otub1 interacts with UbcH5."
23151129
biogrid
No evidence text available
24071738
biogrid
No evidence text available
35927303
biogrid
No evidence text available
32296183
biogrid
No evidence text available
24403071
sparser
"Importantly, Asp88 was found to be the key residue mediating OTUB1 interaction with UbcH5, since mutation of this residue into alanine prevented their binding and abolished OTUB1 effect on p53 xref ."
32201366
biogrid
No evidence text available
26752685
sparser
"In addition, OTUB1-C23-SSG or C204-SSG could realign the OTUB1-bound UBCH5 and free Ub, into a configuration that increases the potency of OTUB1-mediated inhibition of ubiquitination."
37703360
reach
"Instead, OTUB1 binds and inhibits UbcH5 (UBE2D family), the cognate E2 for MDM2 which is a key E3 ligase for P53."
38264570
sparser
"Furthermore, proteomic analyses indicated that OTUB1 binds to E2s of the UBE2D and UBE2E families including the E2 UbcH5 ( xref ; xref )."
22325355
reach
"To see if differences in relative stimulation of DUB activity can be attributed to variations in binding affinities, we determined equilibrium dissociation constants (K d) for OTUB1 binding to UBE2D1, UBE2D2, UBE2D3, UBE2N, and UBE2E1 using isothermal titration calorimetry (ITC)."
32049508
sparser
"This unusual mode of regulation is unlikely to be limited to UBC13 because analysis of OTUB1-associated proteins revealed that OTUB1 binds to E2s of the UBE2D and UBE2E subfamilies."
20725033
sparser
"Further, UbcH5 in turn mediates Otub1 monoubiquitination and this monoubiquitination facilitates the Otub1 binding to UbcH5 and likely inhibits ubiquitin chain transfer [ xref ], providing a mechanism underlying the Otub1 inhibition of E2 activity."
28035068
reach
"Moreover, we demonstrated the endogenous interactions between OTUB1 and UBE2N or UBE2D1 (XREF_FIG)."
24071738
biogrid
No evidence text available
22124327
reach
"Consistently, Otub1 interacts with UbcH5."
23151129
biogrid
No evidence text available
32049508
reach
"Further, UbcH5 in turn mediates Otub1 monoubiquitination and this monoubiquitination facilitates the Otub1 binding to UbcH5 and likely inhibits ubiquitin chain transfer [XREF_BIBR], providing a mechanism underlying the Otub1 inhibition of E2 activity."
28035068
sparser
"Instead, OTUB1 binds and inhibits UbcH5 (UBE2D family), the cognate E2 for MDM2 which is a key E3 ligase for P53."
38264570
reach
"To address this possible scenario, we determined whether OTUB1-SSG affected the interaction between OTUB1 and UBCH5A that requires the N-terminal region containing C23 (43)."
37703360
reach
"To understand how S-glutathionylation affects the OTUB1-UBCH5A complex, we created three different in silico models of OTUB1 in conjunction with Ub-charged UBCH5A and free Ub."
37703360
reach
"GLRX also decreases the interaction between OTUB1 and UBCH5A (Fig. 7D)."
37703360
biogrid
No evidence text available
33961781
reach
"In addition, OTUB1-C23-SSG or C204-SSG could realign the OTUB1-bound UBCH5 and free Ub, into a configuration that increases the potency of OTUB1-mediated inhibition of ubiquitination."
37703360