IndraLab

Statements

USP16 binds Flag. 8 / 8
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"To test this, we transfected Flag-USP16 or the 2A mutant into HeLa cells and isolated USP16 by Flag IP."
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"For this purpose, we cotransfected HeLa cells with Myc-OGT and Flag-USP16 and performed co-immunoprecipitation (co-IP) assays with the anti-Flag antibody ( xref A )."
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"Labelling experiments were performed with HeLa cells lysate overexpressing Flag-USP5, Flag-USP7, Flag-USP15 or Flag-USP16 and their catalytically inactive versions ( xref )."
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"In xref , it is shown that only Flag-USP16 reacts with M20, while all the other DUBs are labelled only by the wild-type probe."
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"First, we transfected Flag-USP16, HA-Ub, and Myc-OGT into the cells ( xref A ) and used Noc to synchronize them into the M phase."
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"To determine whether the change in the subcellular localization of USP16 is due to impaired interaction with Crm1, we transfected HeLa cells with Flag-USP16 and HA-Crm1."
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"The interaction was also confirmed by revealing that GST-OGT, but not GST itself, pulled down Flag-USP16 expressed in HeLa cells ( xref D )."
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"Furthermore, USP16 protein was detected when Flag-c-Myc was immunoprecipitated by Flag antibody, and inversely c-Myc was detected when Flag-USP16 was immunoprecipitated in PC3 cells (Fig. xref c and d)."
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