IndraLab

Statements

OTUD7B binds Flag. 6 / 6
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"Concordantly, the ubiquitination level of N1ICD was dramatically attenuated in the Flag-OTUD7B group, whereas the N1ICD ubiquitination level was restored in the Flag-OTUD7B-C194S group (Fig. xref )."
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"Upon H 2 O 2 treatment, we found that exogenously expressed C-terminally epitope-tagged human Cezanne (Cezanne-Flag) was labeled by DCP-Bio1 in cell lysates ( xref ), suggesting that Cezanne was oxidized to the sulfenic acid intermediate in response to oxidative stress."
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"Further IP experiments showed that both Flag-OTUD7B and Flag-p53 were able to co-precipitate the endogenous forms of p53 and OTUD7B in 293T cells, respectively (Figure xref E)."
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"Flag-USP4, Flag-SENP2, Flag-USP22, Flag-OTUD7B, Flag-USP11, Flag-UCHL1, Flag-USP5WT GST-USP5 WT , GST-USP5 C119A , His-OCT4, HA-Ub, HA-Ub-Lys0, HA-Ub-Lys48, and HA-Ub-Lys63 overexpression plasmids wer[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The N1ICD level was profoundly upregulated in the OTUD7B wild-type (Flag-OTUD7B) group, but it was restored by the catalytically inactive mutant C194S of OTUD7B (Flag-OTUD7B-C194S) (Fig. xref )."
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"Similarly, upon transfecting HEK293T cells with Flag-OTUD7B, His- HNF4α, and HA-Ub, we observed that OTUD7B stabilized HNF4α protein levels (Fig. xref E)."
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