IndraLab

Statements

USP28 is ubiquitinated. 9 / 9
| 9
sparser
"As expected, use of this mutant resulted in a loss of USP28 ubiquitination by DTX3L ( xref F)."
40703443
sparser
"LDHA ablation also rescued USP28 ubiquitination that was inhibited by epinephrine ( xref )."
30688660
sparser
"In the third mutant (Ub-K63), only K63 remains intact allowing only K63-linked polyubiquitin chains, and DTX3L was also able to ubiquitinate USP28 with this mutant ( xref F)."
40703443
sparser
"Next, we used mass spectrometry to identify the sites within USP28 C171A that become ubiquitinated in the presence of DTX3L. In total, 28 lysins were found to be modified by DTX3L and those ubiquitination sites were distributed over the whole protein without any domain preference ( xref G)."
40703443
sparser
"Similarly, when we performed ubiquitination assays for USP28 in cells that either overexpressed wild type USP28 or the inactive USP28 C171A along with DTX3L, we found that DTX3L promoted ubiquitination of USP28."
40703443
sparser
"To further investigate the role of DTX3L’s E3 ligase activity in the regulation of USP28, we conducted additional experiments using an E3 ligase-deficient mutant of DTX3L. We used this mutant in both cell assays and with recombinant proteins and demonstrated that the inactive DTX3L does not ubiquitinate USP28 ( xref )."
40703443
sparser
"Our results show that while DYRK2 acts as a scaffold to facilitate USP28 ubiquitination, it also directly phosphorylates USP28, affecting its regulation."
40858801
sparser
"The assay shows that DTX3L is able to ubiquitinate USP28 in the presence of this mutant."
40703443
sparser
"Consistently, DYRK2, regardless of its kinase activity, enhanced USP28 ubiquitination (Fig.  xref ) and USP28-T516A/S517A/S518A mutant degradation (Fig.  xref )."
40858801