IndraLab

"Interestingly, UCH37 also associates in the nucleus with the human Ino80 chromatin-remodeling complex, where it is held in an inactive state compared to the free enzyme( xref )."

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"Uch37 is bound to hINO80 via its C-terminal tail and the N-terminal domain of NFRKB."

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"Further studies have suggested that UCH37 is associated with the human Ino80 chromatin-remodeling complex (hINO80) in the nucleus and can be activated via transient association of 19S regulatory parti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Our dissection of interactions between hINO80 and Uch37 shows that one polypeptide, NFRKB, has elements that can inhibit and activate deubiquitinating activity."

"In contrast, INO80 associated Uch37 or Uch37 in complex with the NFRKB 1-465 fragment is either inhibited (as assessed by UbVS reactivity) or held in a relatively inactive state similar to that of free Uch37 (as assessed by its ability to hydrolyze UbAMC)."

"Considering that INO80 functions in transcription and DNA repair through chromatin remodeling [65], histones and transcription factors are among likely candidates for substrates of INO80 bound Uch37."

"The transient association of the INO80 complex with UCH37 regulates the deubiquitylating activity of this subunit."

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"We can not, however, exclude the possibility that assembly of Uch37 into hINO80 affects DUB activity by a different mechanism since our attempts to measure the binding of ubiquitin to Uch37 within hIN[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"And UCH-L5 also interacts with NFRKB in INO80 complex, but it remains unknown that whether UCH-L5 interacts with other components of INO80 complex or not."

"In the nucleus, UCHL5 is also associated with human Ino80 chromatin-remodeling complex and kept in inactive state, and then activated by transient interaction of the Ino80 complex with proteasome, suggesting that it may cooperate to regulate transcription or DNA repair xref ."

"For example, as mentioned above, certain transcriptional activators are ubiquitylated to regulate their stability or activity and INO80 bound Uch37 could regulate transcription through these mediators."

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"UCH-L1 possesses deubiquitinating activity and dimerization-dependent ubiquitin ligase activity, and functions as a mono-ubiquitin stabilizer; UCH-L3 does both deubiquitinating and deneddylating activity, except dimerization or ligase activity, and unlike UCH-L1, can interact with Lys48-linked Ub dimers to protect it from degradation and in the meanwhile to inhibit its hydrolase activity; UCH37 is responsible for the deubiquitinating activity in the 19S proteasome regulatory complex, and as indicated by the recent study, UCH37 is also associated with the human Ino80 chromatin-remodeling complex (hINO80) in the nucleus and can be activated via transient association of 19S regulatory particle- or proteasome-bound hRpn13 with hINO80; BAP1, binding to the wild-type BRCA1 RING finger domain, is regarded as a tumor suppressor, but for such suppressing activity, as demonstrated otherwise, both deubiquitinating activity and nucleus localization are required."

"Considering that INO80 functions in transcription and DNA repair through chromatin remodeling [65] , histones and transcription factors are among likely candidates for substrates of INO80-bound Uch37[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Here we show that in the nucleus Uch37 is also associated with the human Ino80 chromatin-remodeling complex (hINO80)."

"It is important to note, however, that although NFRKB was the only hINO80 subunit that interacted with Uch37 in our yeast two-hybrid assays, it is likely that Uch37 interacts with other hINO80 subunits in the context of the intact complex."

"Yao et al. xref demonstrated that Uch37 also associates with the Ino80 chromatin-remodeling complex (Ino80 complex), which is involved in DNA repair and transcriptional regulation."

"For example, as mentioned above, certain transcriptional activators are ubiquitylated to regulate their stability or activity ( xref ) and INO80-bound Uch37 could regulate transcription through these mediators."

"Considering that INO80 functions in transcription and DNA repair through chromatin remodeling [ xref ], histones and transcription factors are among likely candidates for substrates of INO80-bound Uch37."

"Recent studies have suggested that in the nucleus UCH37 is also associated with the human Ino80 chromatin-remodeling complex (hINO80), and that it binds to hINO80 via its C-terminal tail and N-termina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Thus, it is assumed that in the nucleus UCH37 interacts with two complexes, the 19S proteasome regulatory particle and hINO80 complex [36,37] ."

"Our dissection of interactions between hINO80 and Uch37 shows that one polypeptide, NFRKB, has elements that can inhibit and activate deubiquitinating activity."